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Literature summary for 3.2.1.81 extracted from
- The endo-beta-agarases AgaA and AgaB from the marine bacterium Zobellia galactanivorans: two paralogue enzymes with different molecular organizations and catalytic behaviours (2005), Biochem. J., 385, 703-713.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| AgaA, AgaB and truncated form of AgaA referred to as AgaAc with His tag, overexpression in Escherichia coli | Zobellia galactanivorans |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 1 | - |
liquid-phase agarose | AgaB | Zobellia galactanivorans | |
| 2 | - |
liquid-phase agarose | AgaAc | Zobellia galactanivorans |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| extracellular | - |
Zobellia galactanivorans | - |
- |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 13400 | - |
AgaAc, calculated by sequence | Zobellia galactanivorans |
| 32150 | - |
AgaAc, ESI MS | Zobellia galactanivorans |
| 40010 | - |
AgaB, ESI MS | Zobellia galactanivorans |
| 353000 | - |
AgaB, calculated by sequence | Zobellia galactanivorans |
| 539000 | - |
AgaA, calculated by sequence | Zobellia galactanivorans |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Zobellia galactanivorans | - |
Dsij DSMZ 12802 and CIP 106680 | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| ammonium sulfate precipitation, Sepharose CL6B, anion-exchange Mono Q HR5, Ni-NTA column, AgaB further gel filtration with Sephacryl S200 | Zobellia galactanivorans |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.1 | - |
AgaB, 1 ml of 0.125% melted agarose, 44°C, pH 7.5 | Zobellia galactanivorans |
| 0.16 | - |
AgaAc, 1 ml of 0.125% melted agarose, 44°C, pH 7.5 | Zobellia galactanivorans |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| agarose + H2O | - |
Zobellia galactanivorans | ? | - |
? |  |
| liquid-phase agarose + H2O | - |
Zobellia galactanivorans | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | AgaB, gel filtration and ESI MS | Zobellia galactanivorans |
| monomer | AgaA, gel filtration and ESI MS | Zobellia galactanivorans |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| AgaA | - |
Zobellia galactanivorans |
| AgaAc | - |
Zobellia galactanivorans |
| AgaB | - |
Zobellia galactanivorans |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6 | - |
AgaAc | Zobellia galactanivorans |
| 7 | - |
AgaB | Zobellia galactanivorans |
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Release 2023.1 (January 2023)
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