Cloned (Comment) | Organism |
---|---|
AgaA, AgaB and truncated form of AgaA referred to as AgaAc with His tag, overexpression in Escherichia coli | Zobellia galactanivorans |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1 | - |
liquid-phase agarose | AgaB | Zobellia galactanivorans | |
2 | - |
liquid-phase agarose | AgaAc | Zobellia galactanivorans |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
extracellular | - |
Zobellia galactanivorans | - |
- |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
13400 | - |
AgaAc, calculated by sequence | Zobellia galactanivorans |
32150 | - |
AgaAc, ESI MS | Zobellia galactanivorans |
40010 | - |
AgaB, ESI MS | Zobellia galactanivorans |
353000 | - |
AgaB, calculated by sequence | Zobellia galactanivorans |
539000 | - |
AgaA, calculated by sequence | Zobellia galactanivorans |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Zobellia galactanivorans | - |
Dsij DSMZ 12802 and CIP 106680 | - |
Purification (Comment) | Organism |
---|---|
ammonium sulfate precipitation, Sepharose CL6B, anion-exchange Mono Q HR5, Ni-NTA column, AgaB further gel filtration with Sephacryl S200 | Zobellia galactanivorans |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.1 | - |
AgaB, 1 ml of 0.125% melted agarose, 44°C, pH 7.5 | Zobellia galactanivorans |
0.16 | - |
AgaAc, 1 ml of 0.125% melted agarose, 44°C, pH 7.5 | Zobellia galactanivorans |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
agarose + H2O | - |
Zobellia galactanivorans | ? | - |
? | |
liquid-phase agarose + H2O | - |
Zobellia galactanivorans | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | AgaB, gel filtration and ESI MS | Zobellia galactanivorans |
monomer | AgaA, gel filtration and ESI MS | Zobellia galactanivorans |
Synonyms | Comment | Organism |
---|---|---|
AgaA | - |
Zobellia galactanivorans |
AgaAc | - |
Zobellia galactanivorans |
AgaB | - |
Zobellia galactanivorans |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6 | - |
AgaAc | Zobellia galactanivorans |
7 | - |
AgaB | Zobellia galactanivorans |