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Literature summary for 3.2.1.81 extracted from
- Purification and characterization of two different agarose-degrading enzymes (1978), Biochim. Biophys. Acta, 537, 31-43.
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 14000 | - |
alpha2, 2 * 14000, agarase I | Pseudomonas-like bacterium |
| 61000 | - |
gel filtration, beta-agarase IIb | Pseudomonas-like bacterium |
| 63600 | - |
analytical ultracentrifugation, beta-agarase IIb | Pseudomonas-like bacterium |
| 64000 | - |
SDS-PAGE, beta-agarase IIb | Pseudomonas-like bacterium |
| 127000 | - |
gel filtration, beta-agarase I | Pseudomonas-like bacterium |
| 140000 | - |
SDS-PAGE, beta-agarase I | Pseudomonas-like bacterium |
| 210000 | - |
analytical ultracentrifugation, beta-agarase I | Pseudomonas-like bacterium |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| agarose + H2O | Cytophaga sp. | - |
neoagarotetraose | - |
ir |  |
| agarose + H2O | Pseudomonas-like bacterium | - |
neoagarotetraose | - |
ir | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Cytophaga sp. | - |
- |
- |
| Pseudomonas-like bacterium | - |
- |
- |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| glycoprotein | galactose, N-acetylglucosamine and glucosamine | Pseudomonas-like bacterium |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Pseudomonas-like bacterium |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -20°C, at least 6 months | Pseudomonas-like bacterium |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| agarose + H2O | - |
Cytophaga sp. | neoagarotetraose | - |
ir | |
| agarose + H2O | - |
Pseudomonas-like bacterium | neoagarotetraose | - |
ir | |
| neoagarooctaose + H2O | - |
Pseudomonas-like bacterium | neoagarotetraose | beta-agarose IIb | ir | |
| neoagarooctaose + H2O | - |
Cytophaga sp. | neoagarohexaose + neoagarobiose | - |
ir | |
| neoagarooctaose + H2O | - |
Pseudomonas-like bacterium | neoagarohexaose + neoagarobiose | beta-agarase I | ir | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | alpha2, 2 * 14000, agarase I | Pseudomonas-like bacterium |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 38 | - |
beta-agarase I | Pseudomonas-like bacterium |
| 43 | - |
beta-agarase IIb | Pseudomonas-like bacterium |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6.7 | - |
beta-agarase I and IIb | Pseudomonas-like bacterium |
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