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Literature summary for 3.2.1.80 extracted from

  • Shi, Q.; Hou, Y.; Xu, Y.; Moerkeberg Krogh, K.B.R.; Tenkanen, M.
    Enzymatic analysis of levan produced by lactic acid bacteria in fermented doughs (2019), Carbohydr. Polym., 208, 285-293 .
    View publication on PubMed

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
fructan + H2O Paenibacillus amylolyticus
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?
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?

Organism

Organism UniProt Comment Textmining
Paenibacillus amylolyticus
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-
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
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the levanase preparation exhibited an activity of 1116 U/ml towards levan Paenibacillus amylolyticus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
fructan + H2O
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Paenibacillus amylolyticus ?
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?
levan + H2O commercial Erwinia herbicola levan and levan from Leuconostoc mesenteroides DSM 20343-fermented fava bean doughs, combined activity of exoinulinase/levanase Paenibacillus amylolyticus ?
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?
additional information exo-beta-fructosidases, including exoinulinases (EC 3.2.1.80), can nonspecifically hydrolyze both beta-(2->1) and beta-(2->6)-linkages from the non-reducing end of fructooligosaccharides and fructans. In order to develop a specific analysis for levan in food matrices, a Paenibacillus amylolyticus endolevanase (EC 3.2.1.65) is combined with exoinulinase for levan hydrolysis achieving about 80% hydrolysis yield (19.1% for exoinulinase alone), method evaluation, and modelling. The levanase preparation exhibits an activity of 1116 U/ml towards levan and no activity towards inulin as determined by the reducing value method Paenibacillus amylolyticus ?
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?

Synonyms

Synonyms Comment Organism
exoinulinase
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Paenibacillus amylolyticus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
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assay at Paenibacillus amylolyticus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
4
-
-
Paenibacillus amylolyticus