Cloned (Comment) | Organism |
---|---|
expressed in Pichia pastoris | Arctium lappa |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
65666 | - |
x * 65666, calculated from amino acid sequence | Arctium lappa |
80000 | - |
x * 80000, SDS-PAGE | Arctium lappa |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Arctium lappa | - |
- |
- |
Purification (Comment) | Organism |
---|---|
DEAE-Sepharose CL-6B column chromatography, Toyopearl HW55S column chromatography, and Toyopearl HW55S column chromatography | Arctium lappa |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
1.765 | - |
enzyme from supernatant, at pH 6.5 and 30°C | Arctium lappa |
73.4 | - |
after 41.6fold purification, at pH 6.5 and 30°C | Arctium lappa |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
1-kestose + H2O | 100% activity | Arctium lappa | ? | - |
? | |
6-kestose + H2O | 2% activity compared to 1-kestose | Arctium lappa | ? | - |
? | |
burdock root sugar extract + H2O | - |
Arctium lappa | fructose + sucrose + ? | - |
? | |
fructosylnystose + H2O | 97% activity compared to 1-kestose | Arctium lappa | ? | - |
? | |
inulin + H2O | 42% activity compared to 1-kestose | Arctium lappa | ? | - |
? | |
inulobiose + H2O | 12% activity compared to 1-kestose | Arctium lappa | ? | - |
? | |
inulotriose + H2O | 41% activity compared to 1-kestose | Arctium lappa | ? | - |
? | |
additional information | no activity towards levan | Arctium lappa | ? | - |
? | |
additional information | the enzyme preferentially hydrolyzes the beta-2,1 linkage of terminal fructosyl residue of fructan | Arctium lappa | ? | - |
? | |
neokestose + H2O | 7% activity compared to 1-kestose | Arctium lappa | ? | - |
? | |
nystose + H2O | 66% activity compared to 1-kestose | Arctium lappa | ? | - |
? | |
sucrose + H2O | 0.2% activity compared to 1-kestose | Arctium lappa | D-fructose + D-glucose | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 80000, SDS-PAGE | Arctium lappa |
? | x * 65666, calculated from amino acid sequence | Arctium lappa |
Synonyms | Comment | Organism |
---|---|---|
1-FEH | - |
Arctium lappa |
fructan 1-exohydrolase | - |
Arctium lappa |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
stable at temperatures up to 40°C | Arctium lappa |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5.8 | - |
- |
Arctium lappa |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
5.5 | 9 | - |
Arctium lappa |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Arctium lappa | calculated from amino acid sequence | - |
4.9 |