Application | Comment | Organism |
---|---|---|
synthesis | the enzyme preparation is used to hydrolyze pure inulin and raw inulin from Asparagus racemosus for the preparation of a high-fructose syrup | Kluyveromyces marxianus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Ag+ | potent inhibitor below 1 mM | Kluyveromyces marxianus | |
CuSO4 | 1 mM, 47% inhibition | Kluyveromyces marxianus | |
EDTA | 1 mM, 50% inhibition | Kluyveromyces marxianus | |
Fe2(SO4)3 | 1 mM, 38% inhibition | Kluyveromyces marxianus | |
Hg2+ | potent inhibitor below 1mM | Kluyveromyces marxianus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
2.7 | - |
raffinose | pH 5.5, 50°C | Kluyveromyces marxianus | |
3.4 | - |
inulin | pH 5.5, 50°C | Kluyveromyces marxianus | |
25.3 | - |
sucrose | pH 5.5, 50°C | Kluyveromyces marxianus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
extracellular | - |
Kluyveromyces marxianus | - |
- |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | maximal increase in activity: 1.2fold | Kluyveromyces marxianus | |
Mn2+ | maximal increase in activity: 2.4fold | Kluyveromyces marxianus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Kluyveromyces marxianus | - |
- |
- |
Kluyveromyces marxianus Ys-1 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
partial | Kluyveromyces marxianus |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
413 | - |
- |
Kluyveromyces marxianus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
inulin + H2O | - |
Kluyveromyces marxianus | D-glucose + D-fructose + ? | - |
? | |
inulin + H2O | - |
Kluyveromyces marxianus Ys-1 | D-glucose + D-fructose + ? | - |
? | |
raffinose + H2O | hydrolyzed at 69% of the activity with inulin | Kluyveromyces marxianus | beta-D-fructofuranose + O6-alpha-D-galactopyranosyl-alpha-D-glucopyranoside | - |
? | |
raffinose + H2O | hydrolyzed at 69% of the activity with inulin | Kluyveromyces marxianus Ys-1 | beta-D-fructofuranose + O6-alpha-D-galactopyranosyl-alpha-D-glucopyranoside | - |
? | |
sucrose + H2O | hydrolyzed at 177.6% of the activity with inulin | Kluyveromyces marxianus | D-fructose + D-glucose | - |
? | |
sucrose + H2O | hydrolyzed at 177.6% of the activity with inulin | Kluyveromyces marxianus Ys-1 | D-fructose + D-glucose | - |
? |
Synonyms | Comment | Organism |
---|---|---|
exoinulinase | - |
Kluyveromyces marxianus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
- |
Kluyveromyces marxianus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
3 h, stable | Kluyveromyces marxianus |
60 | - |
3 h, 50% loss of activity | Kluyveromyces marxianus |
70 | - |
3 h, 85% loss of activity | Kluyveromyces marxianus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5.5 | - |
- |
Kluyveromyces marxianus |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
5 | 6 | 50°C, 3 h, stable | Kluyveromyces marxianus |