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Literature summary for 3.2.1.80 extracted from

  • Zhang, L.; Zhao, C.; Zhu, D.; Ohta, Y.; Wang, Y.
    Purification and characterization of inulinase from Aspergillus niger AF10 expressed in Pichia pastoris (2004), Protein Expr. Purif., 35, 272-275.
    View publication on PubMed
Search for more literature for 3.2.1.80

Activating Compound

Activating Compound Comment Organism Structure
DDT at 55ºC, pH 4.5, 5 mM, 121% of the relative activity without metal ions Aspergillus niger

Application

Application Comment Organism
nutrition production of ultra high fructose syrup Aspergillus niger

Cloned(Commentary)

Cloned (Comment) Organism
expression in Pichia pastoris Aspergillus niger

Inhibitors

Inhibitors Comment Organism Structure
Al3+ at 55ºC, pH 4.5, 5 mM, 89% inhibition Aspergillus niger
Cu2+ at 55ºC, pH 4.5, 5 mM, 20% inhibition Aspergillus niger
D-glucose at 55ºC, pH 4.5, concentration higher than 2% Aspergillus niger
Fe3+ at 55ºC, pH 4.5, 5 mM, 45% inhibition Aspergillus niger
Hg2+ at 55ºC, pH 4.5, 5 mM, 91% inhibition Aspergillus niger
Mg2+ at 55ºC, pH 4.5, 5 mM, 41% inhibition Aspergillus niger
additional information no inhibited by EDTA and 2-mercaptoethanol Aspergillus niger
Zn2+ at 55ºC, pH 4.5, 5 mM, 8% inhibition Aspergillus niger

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information Michaelis-Menten kinetics Aspergillus niger
2.57
-
 inulin at 55ºC, pH 4.5 Aspergillus niger

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ at 55ºC, pH 4.5, 5 mM, 138% of the relative activity without metal ions Aspergillus niger
Fe2+ at 55ºC, pH 4.5, 5 mM, 123% of the relative activity without metal ions Aspergillus niger
Mn2+ at 55ºC, pH 4.5, 5 mM, 112% of the relative activity without metal ions Aspergillus niger

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
83000
-
 SDS-PAGE Aspergillus niger

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
inulin + 5 H2O Aspergillus niger
-
 5 beta-D-fructofuranose + alpha-D-glucopyranose
-
 ?
inulin + 5 H2O Aspergillus niger AF10
-
 5 beta-D-fructofuranose + alpha-D-glucopyranose
-
 ?

Organism

Organism UniProt Comment Textmining
Aspergillus niger O74641 AF10
-
Aspergillus niger AF10 O74641 AF10
-

Posttranslational Modification

Posttranslational Modification Comment Organism
no modification
-
 Aspergillus niger

Purification (Commentary)

Purification (Comment) Organism
recombinant enzyme, by ammonium sulfate precipitation and anion exchange column, 4.8fold Aspergillus niger

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
156
-
 at 55ºC, pH 4.5 Aspergillus niger

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
inulin + 5 H2O
-
 Aspergillus niger 5 beta-D-fructofuranose + alpha-D-glucopyranose
-
 ?
inulin + 5 H2O
-
 Aspergillus niger AF10 5 beta-D-fructofuranose + alpha-D-glucopyranose
-
 ?
sucrose + H2O
-
 Aspergillus niger beta-D-fructofuranose + alpha-D-glucopyranose
-
 ?
sucrose + H2O
-
 Aspergillus niger AF10 beta-D-fructofuranose + alpha-D-glucopyranose
-
 ?

Synonyms

Synonyms Comment Organism
exo-beta-D-fructosidase
-
 Aspergillus niger
exoinulinase
-
 Aspergillus niger

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
55
-
 pH 5.5 Aspergillus niger

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
4.5
-
 at 55ºC Aspergillus niger