Activating Compound | Comment | Organism | Structure |
---|---|---|---|
DDT | at 55ºC, pH 4.5, 5 mM, 121% of the relative activity without metal ions | Aspergillus niger |
Application | Comment | Organism |
---|---|---|
nutrition | production of ultra high fructose syrup | Aspergillus niger |
Cloned (Comment) | Organism |
---|---|
expression in Pichia pastoris | Aspergillus niger |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Al3+ | at 55ºC, pH 4.5, 5 mM, 89% inhibition | Aspergillus niger | |
Cu2+ | at 55ºC, pH 4.5, 5 mM, 20% inhibition | Aspergillus niger | |
D-glucose | at 55ºC, pH 4.5, concentration higher than 2% | Aspergillus niger | |
Fe3+ | at 55ºC, pH 4.5, 5 mM, 45% inhibition | Aspergillus niger | |
Hg2+ | at 55ºC, pH 4.5, 5 mM, 91% inhibition | Aspergillus niger | |
Mg2+ | at 55ºC, pH 4.5, 5 mM, 41% inhibition | Aspergillus niger | |
additional information | no inhibited by EDTA and 2-mercaptoethanol | Aspergillus niger | |
Zn2+ | at 55ºC, pH 4.5, 5 mM, 8% inhibition | Aspergillus niger |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | Michaelis-Menten kinetics | Aspergillus niger | |
2.57 | - |
inulin | at 55ºC, pH 4.5 | Aspergillus niger |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | at 55ºC, pH 4.5, 5 mM, 138% of the relative activity without metal ions | Aspergillus niger | |
Fe2+ | at 55ºC, pH 4.5, 5 mM, 123% of the relative activity without metal ions | Aspergillus niger | |
Mn2+ | at 55ºC, pH 4.5, 5 mM, 112% of the relative activity without metal ions | Aspergillus niger |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
83000 | - |
SDS-PAGE | Aspergillus niger |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
inulin + 5 H2O | Aspergillus niger | - |
5 beta-D-fructofuranose + alpha-D-glucopyranose | - |
? | |
inulin + 5 H2O | Aspergillus niger AF10 | - |
5 beta-D-fructofuranose + alpha-D-glucopyranose | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Aspergillus niger | O74641 | AF10 | - |
Aspergillus niger AF10 | O74641 | AF10 | - |
Posttranslational Modification | Comment | Organism |
---|---|---|
no modification | - |
Aspergillus niger |
Purification (Comment) | Organism |
---|---|
recombinant enzyme, by ammonium sulfate precipitation and anion exchange column, 4.8fold | Aspergillus niger |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
156 | - |
at 55ºC, pH 4.5 | Aspergillus niger |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
inulin + 5 H2O | - |
Aspergillus niger | 5 beta-D-fructofuranose + alpha-D-glucopyranose | - |
? | |
inulin + 5 H2O | - |
Aspergillus niger AF10 | 5 beta-D-fructofuranose + alpha-D-glucopyranose | - |
? | |
sucrose + H2O | - |
Aspergillus niger | beta-D-fructofuranose + alpha-D-glucopyranose | - |
? | |
sucrose + H2O | - |
Aspergillus niger AF10 | beta-D-fructofuranose + alpha-D-glucopyranose | - |
? |
Synonyms | Comment | Organism |
---|---|---|
exo-beta-D-fructosidase | - |
Aspergillus niger |
exoinulinase | - |
Aspergillus niger |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
55 | - |
pH 5.5 | Aspergillus niger |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
4.5 | - |
at 55ºC | Aspergillus niger |