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Literature summary for 3.2.1.80 extracted from

  • Moriyama, S.; Akimoto, H.; Suetsugu, N.; Kawasaki, S.; Nakamura, T.; Ohta, K.
    Purification and properties of an extracellular exoinulinase from Penicillium sp. strain TN-88 and sequence analysis of the encoding gene (2002), Biosci. Biotechnol. Biochem., 66, 1887-1896.
    View publication on PubMed

Application

Application Comment Organism
additional information fuel production Penicillium sp.
nutrition production of fructose syrup Penicillium sp.

Cloned(Commentary)

Cloned (Comment) Organism
in Escherichia coli Penicillium sp.

Inhibitors

Inhibitors Comment Organism Structure
Ag+ 1.0 mM, pH 5.0, at 30ºC, 100% inhibition Penicillium sp.
Cu2+ 1.0 mM, pH 5.0, at 30ºC, 26% inhibition Penicillium sp.
EDTA 1.0 mM, pH 5.0, at 30ºC, 12% inhibition Penicillium sp.
Fe3+ 1.0 mM, pH 5.0, at 30ºC, 62% inhibition Penicillium sp.
Hg2+ 1.0 mM, pH 5.0, at 30ºC, 100% inhibition Penicillium sp.
N-bromosuccinimide 1.0 mM, pH 5.0, at 30ºC, 100% inhibition Penicillium sp.
p-chloromercuribenzoate 1.0 mM, pH 5.0, at 30ºC, 65% inhibition Penicillium sp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0926
-
inulin at 40ºC, pH 5.0 Penicillium sp.

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular
-
Penicillium sp.
-
-

Metals/Ions

Metals/Ions Comment Organism Structure
Co2+ 1.0 mM, pH 5.0, at 30ºC, 117% of the relative activity without any added compound Penicillium sp.
Mn2+ 1.0 mM, pH 5.0, at 30ºC, 127% of the relative activity without any added compound Penicillium sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
74520
-
calculation from sequence of amino acid Penicillium sp.
81000
-
SDS-PAGE Penicillium sp.

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
inulin + 5 H2O Penicillium sp.
-
5 beta-D-fructofuranose + alpha-D-glucopyranose
-
?
inulin + 5 H2O Penicillium sp. TN-88
-
5 beta-D-fructofuranose + alpha-D-glucopyranose
-
?

Organism

Organism UniProt Comment Textmining
Penicillium sp. Q8J0G1
-
-
Penicillium sp. TN-88 Q8J0G1
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
no modification
-
Penicillium sp.

Purification (Commentary)

Purification (Comment) Organism
by ultrafiltration, DEAE-cellulofine column, Q-Sepharose column and Superdex column, 329fold Penicillium sp.

Source Tissue

Source Tissue Comment Organism Textmining
culture filtrate
-
Penicillium sp.
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
93.8
-
sucrose, at 40ºC, pH 5.0 Penicillium sp.
743
-
inulin, at 40ºC, pH 5.0 Penicillium sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
inulin + 5 H2O
-
Penicillium sp. 5 beta-D-fructofuranose + alpha-D-glucopyranose
-
?
inulin + 5 H2O no activity with bacterial levan Penicillium sp. 5 beta-D-fructofuranose + alpha-D-glucopyranose
-
?
inulin + 5 H2O
-
Penicillium sp. TN-88 5 beta-D-fructofuranose + alpha-D-glucopyranose
-
?
inulin + 5 H2O no activity with bacterial levan Penicillium sp. TN-88 5 beta-D-fructofuranose + alpha-D-glucopyranose
-
?
raffinose + H2O 18% of the activity toward inulin Penicillium sp. beta-D-fructofuranose + O6-alpha-D-galactopyranosyl-alpha-D-glucopyranoside
-
?
raffinose + H2O 18% of the activity toward inulin Penicillium sp. TN-88 beta-D-fructofuranose + O6-alpha-D-galactopyranosyl-alpha-D-glucopyranoside
-
?
sucrose + H2O
-
Penicillium sp. beta-D-fructofuranose + alpha-D-glucopyranose
-
?
sucrose + H2O
-
Penicillium sp. TN-88 beta-D-fructofuranose + alpha-D-glucopyranose
-
?

Synonyms

Synonyms Comment Organism
exoinulinase
-
Penicillium sp.
P-I
-
Penicillium sp.

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
55
-
pH 5.0 Penicillium sp.

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
50 70 pH 5.0, enzyme remains stable up to 50ºC, but it loses activity at 70ºC Penicillium sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
4
-
at 40ºC Penicillium sp.

pH Stability

pH Stability pH Stability Maximum Comment Organism
5 7 at 30ºC Penicillium sp.

pI Value

Organism Comment pI Value Maximum pI Value
Penicillium sp. analytical isoelectric focusing
-
4.9
Penicillium sp. deduction from sequence of amino acid
-
4.9