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Literature summary for 3.2.1.8 extracted from

  • Sharma, K.; Antunes, I.; Rajulapati, V.; Goyal, A.
    Molecular characterization of a first endo-acting beta-1,4-xylanase of family 10 glycoside hydrolase (PsGH10A) from Pseudopedobacter saltans comb. nov (2018), Process Biochem., 70, 79-89 .
No PubMed abstract available
Search for more literature for 3.2.1.8

Application

Application Comment Organism
additional information the enzyme can be used for various biotechnological applications such as for prebiotic xylooligosaccharides and bioethanol production from pretreated agrowaste biomass Pseudopedobacter saltans

Cloned(Commentary)

Cloned (Comment) Organism
gene Pedsa_2704, DNA and amino acid sequence determination and analysis, phylogenetic analysis and tree, recombinant expression of His-tagged enzyme in Escherichia coli strain BL21(DE3), subcloning in Escherichia coli strain TOP10 Pseudopedobacter saltans

Inhibitors

Inhibitors Comment Organism Structure
Ag+ complete inhibition at 2 mM Pseudopedobacter saltans
Ba2+ 42% inhibition at 2 mM Pseudopedobacter saltans
Co2+ 39% inhibition at 2 mM Pseudopedobacter saltans
Cu2+ 85% inhibition at 2 mM Pseudopedobacter saltans
EDTA 36% inhibition at 2 mM Pseudopedobacter saltans
guanidine hydrochloride 84% inhibition at 2 M Pseudopedobacter saltans
Hg2+ complete inhibition at 2 mM Pseudopedobacter saltans
Li+ 54% inhibition at 2 mM Pseudopedobacter saltans
Ni2+ 48% inhibition at 2 mM Pseudopedobacter saltans
Pb2+ 65% inhibition at 2 mM Pseudopedobacter saltans
SDS 55% inhibition at 0.25% w/v Pseudopedobacter saltans
Urea 80% inhibition at 0.5 M Pseudopedobacter saltans
Zn2+ 51% inhibition at 2 mM Pseudopedobacter saltans

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information Michaelis-Menten and Lineweaver-Burk kinetics, Km for beechwood xylan is 6.2 mg/ml for birchwood 3.9 mg/ml, and for xylan MW 2000-30000 5.1 mg/ml Pseudopedobacter saltans

Metals/Ions

Metals/Ions Comment Organism Structure
Mn2+ activates by 29% at 2 mM Pseudopedobacter saltans

Organism

Organism UniProt Comment Textmining
Pseudopedobacter saltans F0S6Y3 i.e. Pedobacter saltans
-
Pseudopedobacter saltans ATCC 51119 F0S6Y3 i.e. Pedobacter saltans
-
Pseudopedobacter saltans DSM 12145 F0S6Y3 i.e. Pedobacter saltans
-
Pseudopedobacter saltans JCM 21818 F0S6Y3 i.e. Pedobacter saltans
-
Pseudopedobacter saltans LMG 10337 F0S6Y3 i.e. Pedobacter saltans
-
Pseudopedobacter saltans NBRC 100064 F0S6Y3 i.e. Pedobacter saltans
-
Pseudopedobacter saltans NCIMB 13643 F0S6Y3 i.e. Pedobacter saltans
-

Purification (Commentary)

Purification (Comment) Organism
recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography and dialysis Pseudopedobacter saltans

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
8
-
 purified recombinant His-tagged enzyme, pH 6.0, 40°C, substrate oat spelt xylan Pseudopedobacter saltans
12.7
-
 purified recombinant His-tagged enzyme, pH 6.0, 40°C, substrate insoluble wheat arabinoxylan Pseudopedobacter saltans
18.7
-
 purified recombinant His-tagged enzyme, pH 6.0, 40°C, substrate insoluble rye arabinoxylan Pseudopedobacter saltans
22.3
-
 purified recombinant His-tagged enzyme, pH 6.0, 40°C, substrate soluble wheat arabinoxylan Pseudopedobacter saltans
25.4
-
 purified recombinant His-tagged enzyme, pH 6.0, 40°C, substrate soluble rye arabinoxylan Pseudopedobacter saltans
37.3
-
 purified recombinant His-tagged enzyme, pH 6.0, 40°C, substrate birchwood xylan Pseudopedobacter saltans
47
-
 purified recombinant His-tagged enzyme, pH 6.0, 40°C, substrate 4-O methyl-glucuronoxylan Pseudopedobacter saltans
57.1
-
 purified recombinant His-tagged enzyme, pH 6.0, 40°C, substrate xylan with MW of 20000-30000 Pseudopedobacter saltans
59.7
-
 purified recombinant His-tagged enzyme, pH 6.0, 40°C, substrate beechwood xylan Pseudopedobacter saltans

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-O methyl-glucuronoxylan + H2O moderate activity Pseudopedobacter saltans ?
-
 ?
beechwood xylan + H2O best substrate Pseudopedobacter saltans xylose + xylobiose + xylotetraose
-
 ?
birchwood xylan + H2O moderate activity Pseudopedobacter saltans ?
-
 ?
birchwood xylan + H2O moderate activity Pseudopedobacter saltans NBRC 100064 ?
-
 ?
birchwood xylan + H2O moderate activity Pseudopedobacter saltans JCM 21818 ?
-
 ?
birchwood xylan + H2O moderate activity Pseudopedobacter saltans NCIMB 13643 ?
-
 ?
birchwood xylan + H2O moderate activity Pseudopedobacter saltans ATCC 51119 ?
-
 ?
birchwood xylan + H2O moderate activity Pseudopedobacter saltans LMG 10337 ?
-
 ?
birchwood xylan + H2O moderate activity Pseudopedobacter saltans DSM 12145 ?
-
 ?
additional information enzyme PsGH10A hydrolyzes products of xylan and substituted xylan indicate production of series of short-chain xylooligosaccharides and arabinoxylo-saccharides, substrate specificity, overview Pseudopedobacter saltans ?
-
 ?
additional information enzyme PsGH10A hydrolyzes products of xylan and substituted xylan indicate production of series of short-chain xylooligosaccharides and arabinoxylo-saccharides, substrate specificity, overview Pseudopedobacter saltans NBRC 100064 ?
-
 ?
additional information enzyme PsGH10A hydrolyzes products of xylan and substituted xylan indicate production of series of short-chain xylooligosaccharides and arabinoxylo-saccharides, substrate specificity, overview Pseudopedobacter saltans JCM 21818 ?
-
 ?
additional information enzyme PsGH10A hydrolyzes products of xylan and substituted xylan indicate production of series of short-chain xylooligosaccharides and arabinoxylo-saccharides, substrate specificity, overview Pseudopedobacter saltans NCIMB 13643 ?
-
 ?
additional information enzyme PsGH10A hydrolyzes products of xylan and substituted xylan indicate production of series of short-chain xylooligosaccharides and arabinoxylo-saccharides, substrate specificity, overview Pseudopedobacter saltans ATCC 51119 ?
-
 ?
additional information enzyme PsGH10A hydrolyzes products of xylan and substituted xylan indicate production of series of short-chain xylooligosaccharides and arabinoxylo-saccharides, substrate specificity, overview Pseudopedobacter saltans LMG 10337 ?
-
 ?
additional information enzyme PsGH10A hydrolyzes products of xylan and substituted xylan indicate production of series of short-chain xylooligosaccharides and arabinoxylo-saccharides, substrate specificity, overview Pseudopedobacter saltans DSM 12145 ?
-
 ?
oat spelt xylan + H2O low activity Pseudopedobacter saltans ?
-
 ?
oat spelt xylan + H2O low activity Pseudopedobacter saltans NBRC 100064 ?
-
 ?
oat spelt xylan + H2O low activity Pseudopedobacter saltans JCM 21818 ?
-
 ?
oat spelt xylan + H2O low activity Pseudopedobacter saltans NCIMB 13643 ?
-
 ?
oat spelt xylan + H2O low activity Pseudopedobacter saltans ATCC 51119 ?
-
 ?
oat spelt xylan + H2O low activity Pseudopedobacter saltans LMG 10337 ?
-
 ?
oat spelt xylan + H2O low activity Pseudopedobacter saltans DSM 12145 ?
-
 ?
rye arabinoxylan + H2O soluble and insoluble, the latter is less active, moderate to lower activity Pseudopedobacter saltans ?
-
 ?
wheat arabinoxylan + H2O soluble and insoluble, the latter is less active, moderate to lower activity Pseudopedobacter saltans ?
-
 ?
wheat arabinoxylan + H2O soluble and insoluble, the latter is less active, moderate to lower activity Pseudopedobacter saltans NBRC 100064 ?
-
 ?
wheat arabinoxylan + H2O soluble and insoluble, the latter is less active, moderate to lower activity Pseudopedobacter saltans JCM 21818 ?
-
 ?
wheat arabinoxylan + H2O soluble and insoluble, the latter is less active, moderate to lower activity Pseudopedobacter saltans NCIMB 13643 ?
-
 ?
wheat arabinoxylan + H2O soluble and insoluble, the latter is less active, moderate to lower activity Pseudopedobacter saltans ATCC 51119 ?
-
 ?
wheat arabinoxylan + H2O soluble and insoluble, the latter is less active, moderate to lower activity Pseudopedobacter saltans LMG 10337 ?
-
 ?
wheat arabinoxylan + H2O soluble and insoluble, the latter is less active, moderate to lower activity Pseudopedobacter saltans DSM 12145 ?
-
 ?
xylan + H2O molecular weight of 20000-30000, high activity Pseudopedobacter saltans ?
-
 ?
xylan + H2O molecular weight of 20000-30000, high activity Pseudopedobacter saltans NBRC 100064 ?
-
 ?
xylan + H2O molecular weight of 20000-30000, high activity Pseudopedobacter saltans JCM 21818 ?
-
 ?
xylan + H2O molecular weight of 20000-30000, high activity Pseudopedobacter saltans NCIMB 13643 ?
-
 ?
xylan + H2O molecular weight of 20000-30000, high activity Pseudopedobacter saltans ATCC 51119 ?
-
 ?
xylan + H2O molecular weight of 20000-30000, high activity Pseudopedobacter saltans LMG 10337 ?
-
 ?
xylan + H2O molecular weight of 20000-30000, high activity Pseudopedobacter saltans DSM 12145 ?
-
 ?

Subunits

Subunits Comment Organism
? x * 43000, recombinant His-tagged enzyme, SDS-PAGE Pseudopedobacter saltans

Synonyms

Synonyms Comment Organism
beta-1,4-xylanase
-
 Pseudopedobacter saltans
endo-acting beta-1,4-xylanase
-
 Pseudopedobacter saltans
Pedsa_2704
-
 Pseudopedobacter saltans
PsGH10A
-
 Pseudopedobacter saltans

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
40
-
-
 Pseudopedobacter saltans

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
10 55 recombinant enzyme, activity range, profile overview Pseudopedobacter saltans

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
40
-
 purified recombinant enzyme, pH 6.0, 90 min, stable up to Pseudopedobacter saltans
45
-
 purified recombinant enzyme, pH 6.0, 90 min, loss of 50% activity Pseudopedobacter saltans
50
-
 purified recombinant enzyme, pH 6.0, 90 min, loss of 70% activity Pseudopedobacter saltans
60
-
 purified recombinant enzyme, pH 6.0, 90 min, inactivation Pseudopedobacter saltans

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
36.8
-
 birchwood xylan His-tagged enzyme, pH 6.0, 40°C Pseudopedobacter saltans
45.9
-
 xylan His-tagged enzyme, pH 6.0, 40°C, substrate xylan MW 20000-30000 Pseudopedobacter saltans
52.2
-
 beechwood xylan His-tagged enzyme, pH 6.0, 40°C Pseudopedobacter saltans

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6
-
-
 Pseudopedobacter saltans

pH Range

pH Minimum pH Maximum Comment Organism
5 9 recombinant enzyme, activity range, profile overview Pseudopedobacter saltans

pH Stability

pH Stability pH Stability Maximum Comment Organism
4.5
-
 purified recombinant enzyme, 25°C, 90 min, 25% activity remaining Pseudopedobacter saltans
5.5
-
 purified recombinant enzyme, 25°C, 90 min, 50% activity remaining Pseudopedobacter saltans
6 8 purified recombinant enzyme, 25°C, 90 min, over 80% activity remaining Pseudopedobacter saltans
8.5
-
 purified recombinant enzyme, 25°C, 90 min, 50% activity remaining Pseudopedobacter saltans
9
-
 purified recombinant enzyme, 25°C, 90 min, 25% activity remaining Pseudopedobacter saltans

General Information

General Information Comment Organism
evolution the enzyme belongs to the glycoside hydrolase family 10, GH10 Pseudopedobacter saltans