Activating Compound | Comment | Organism | Structure |
---|---|---|---|
EDTA | - |
Acetivibrio thermocellus |
Cloned (Comment) | Organism |
---|---|
CtXynGH30, recombinant overexpression in Escherichia coli strain BL21 | Acetivibrio thermocellus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
2-mercaptoethanol | - |
Acetivibrio thermocellus | |
Ca2+ | slight inhibition | Acetivibrio thermocellus | |
Co2+ | - |
Acetivibrio thermocellus | |
Cu2+ | - |
Acetivibrio thermocellus | |
Fe2+ | - |
Acetivibrio thermocellus | |
Hg2+ | complete inhibition at 1 mM | Acetivibrio thermocellus | |
Mg2+ | slight inhibition | Acetivibrio thermocellus | |
NaCl | - |
Acetivibrio thermocellus | |
Ni2+ | - |
Acetivibrio thermocellus | |
SDS | - |
Acetivibrio thermocellus | |
Urea | - |
Acetivibrio thermocellus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | Vmax and Km are 40.52 U/mg and 2.2 mg/ml, respectively, with beechwood xylan as substrate | Acetivibrio thermocellus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Li+ | activates | Acetivibrio thermocellus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Acetivibrio thermocellus | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-O-methyl glucuronoxylan + H2O | - |
Acetivibrio thermocellus | ? | - |
? | |
arabinan + H2O | from sugar beet, low activity | Acetivibrio thermocellus | ? | - |
? | |
arabinogalactan + H2O | low activity | Acetivibrio thermocellus | ? | - |
? | |
arabinoxylan + H2O | from rye or wheat, low activity | Acetivibrio thermocellus | ? | - |
? | |
beechwood xylan + H2O | best substrate, TLC analysis of CtXynGH30 hydrolysis products from beechwood xylan. CtXynGH30 shows the release of a series of higher xylooligosaccharides, overview | Acetivibrio thermocellus | ? | - |
? | |
birchwood xylan + H2O | high activity | Acetivibrio thermocellus | ? | - |
? | |
galactomannan + H2O | from Konjac, low activity | Acetivibrio thermocellus | ? | - |
? | |
galactomannan + H2O | from locust bean, low activity | Acetivibrio thermocellus | ? | - |
? | |
additional information | CtXynGH30 shows the release of a series of higher xylooligosaccharides, implying the endo-acting cleavageby the enzyme. No activity on 4-nitrophenyl-beta-D-xylopyranoside indicating that CtXynGH30 lacks exo-cleaving activity. No activity with carob galactomannan, galactan (lupin), beta-D-glucan (barley), carboxy methylcellulose, pecticgalactan (apple), pecticgalactan (Citrus), pecticgalactan (lupin), rhamnogalacturonan, curdlan, pullulan, and pustulan | Acetivibrio thermocellus | ? | - |
? | |
oat spelt xylan + H2O | - |
Acetivibrio thermocellus | ? | - |
? | |
xyloglucan + H2O | low activity | Acetivibrio thermocellus | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 60000, recombinant enzyme, SDS-PAGE, x * 61200, about, sequence calculation | Acetivibrio thermocellus |
Synonyms | Comment | Organism |
---|---|---|
CtXynGH30 | - |
Acetivibrio thermocellus |
family 30 glycoside hydrolase subfamily 8 glucuronoxylan endo-beta-1,4-xylanase | - |
Acetivibrio thermocellus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
70 | - |
- |
Acetivibrio thermocellus |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | 85 | over 50% of maximal activity within this range, profile overview | Acetivibrio thermocellus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
2008.3 | - |
beechwood xylan | pH 6.0, 37°C | Acetivibrio thermocellus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6 | - |
- |
Acetivibrio thermocellus |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
2.5 | 11.5 | activity range, profile overview | Acetivibrio thermocellus |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
4 | 11 | completely stable at pH 7.0, over 50% activity remaining at pH 4.0-11.0, inactivation at pH 12.0 and pH below 2.0 | Acetivibrio thermocellus |
General Information | Comment | Organism |
---|---|---|
evolution | CtXynGH30 is a family 30 glycoside hydrolase subfamily 8 glucuronoxylan endo-beta-1,4-xylanase | Acetivibrio thermocellus |