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Literature summary for 3.2.1.8 extracted from
- Heterologous expression, purification and characterization of a highly thermolabile endoxylanase from the Antarctic fungus Cladosporium sp (2018), Fungal Biol., 122, 875-882 .
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Pichia pastoris | Cladosporium sp. |
| gene xynA, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic tree, recombinant expression of His- and c-myc-tagged enzyme in Pichia pastoris strain GS115, the enzyme is secreted | Cladosporium sp. |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 34400 | - |
calculated from sequence | Cladosporium sp. |
| 37000 | - |
SDS-PAGE | Cladosporium sp. |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Cladosporium sp. | A0A346PXS9 | - |
- |
| Cladosporium sp. | A0A346PXS9 | isolated from Antarctica | - |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| additional information | no N- or O-glycosylations sites are detected by bioinformatics tools | Cladosporium sp. |
| no glycoprotein | no N- or O-glycosylations sites are detected by bioinformatic tools | Cladosporium sp. |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Cladosporium sp. |
| recombinant extracellular His- and c-myc-tagged enzyme from Pichia pastoris strain GS115 by anion exchange and cation exchange chromatography | Cladosporium sp. |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| mycelium | Cladosporium sp. is grown on CM medium at 15°C and 180 rpm during 72 h | Cladosporium sp. | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 728 | - |
purified extracellular recombinant enzyme, pH 6.0, 50°C, substrate rye arabinoxylan | Cladosporium sp. |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| arabinoxylan + H2O | the enzyme is most active on xylans with high content of arabinose (rye arabinoxylan and wheat arabinoxylan) than on xylans with low content of arabinose (oat spelts xylan, birchwood xylan and beechwood xylan). It releases xylooligosaccharides but not xylose, indicating that XynA is an endoxylanase | Cladosporium sp. | xylobiose + xylotriose + xylotetraose + ? | - |
? |  |
| beechwood xylan + H2O | 62.6% activity compared to rye arabinoxylan | Cladosporium sp. | ? | - |
? | |
| birchwood xylan + H2O | 32.4% activity compared to rye arabinoxylan | Cladosporium sp. | ? | - |
? | |
| additional information | the enzyme is more active on xylans with high content of arabinose (rye arabinoxylan and wheat arabinoxylan) than on xylans with low content of arabinose (oat spelts xylan, birchwood xylan and beechwood xylan) | Cladosporium sp. | ? | - |
? | |
| oat spelt xylan + H2O | 61.9% activity compared to rye arabinoxylan | Cladosporium sp. | ? | - |
? | |
| rye arabinoxylan + H2O | best substrate | Cladosporium sp. | ? | - |
? | |
| wheat arabinoxylan + H2O | 92.0% activity compared to rye arabinoxylan | Cladosporium sp. | ? | - |
? | |
| xylan + H2O | the enzyme is most active on xylans with high content of arabinose (rye arabinoxylan and wheat arabinoxylan) than on xylans with low content of arabinose (oat spelts xylan, birchwood xylan and beechwood xylan). It releases xylooligosaccharides but not xylose, indicating that XynA is an endoxylanase | Cladosporium sp. | xylobiose + xylotriose + xylotetraose + ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 34441, sequence calculation, x * 37000, recombinant extracellular His- and c-myc-tagged enzyme, SDS-PAGE | Cladosporium sp. |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| beta-xylanase | - |
Cladosporium sp. |
| endoxylanase | - |
Cladosporium sp. |
| XynA | - |
Cladosporium sp. |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | - |
- |
Cladosporium sp. |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 20 | 60 | 20°C: about 40% of maximal activity, 60°C: about 35% of maximal activity | Cladosporium sp. |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 5 | 25 | purified recombinant enzyme, 3 h, over 90% activity remaining | Cladosporium sp. |
| 30 | - |
3 h, 50% loss of activity | Cladosporium sp. |
| 30 | - |
purified recombinant enzyme, 3 h, loss of 50% activity | Cladosporium sp. |
| 35 | - |
1 h, 50% loss of activity | Cladosporium sp. |
| 35 | - |
purified recombinant enzyme, 1 h, loss of 50% activity | Cladosporium sp. |
| 40 | - |
20-30 min, complete inactivation | Cladosporium sp. |
| 40 | - |
purified recombinant enzyme, 20-30 min, complete inactivation | Cladosporium sp. |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6 | 7 | - |
Cladosporium sp. |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 5 | 8 | pH 5.0: about 60% of maximal activity, pH 8.0: about 35% of maximal activity | Cladosporium sp. |
General Information
| General Information | Comment | Organism |
|---|---|---|
| evolution | the enzyme belongs to the glycoside hydrolase family 10, GH10 | Cladosporium sp. |
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