Application | Comment | Organism |
---|---|---|
food industry | the ability of the enzym to produce xylobiose from agricultural and forestry residues proves that it is an excellent candidate enzyme in prebiotic and alternative sweetener industries | Streptomyces ipomoeae |
Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Streptomyces ipomoeae |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
beech wood xylan | Km-value: 1.14 g/l, pH 6.5, 75°C | Streptomyces ipomoeae |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
44000 | - |
SDS-PAGE | Streptomyces ipomoeae |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Streptomyces ipomoeae | MK331807 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Streptomyces ipomoeae |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
197.75 | - |
substrate: beech wood xylan, pH 6.5, 75°C | Streptomyces ipomoeae |
601.32 | - |
substrate: wheat arabinoxylan, pH 6.5, 75°C | Streptomyces ipomoeae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
beech wood xylan + H2O | hydrolysis of beechwood xylan in endo-action mode releasing xylobiose as its main end product | Streptomyces ipomoeae | xylobiose + ? | - |
? | |
additional information | the xylobiose yield from 10 g corncob and moso bamboo reached 1.123 g and 0.229 g, respectively, at pH 6.5 and 70 °C. High ratios of xylobiose in the xylose-based product of about 85% are obtained from corncob, moso bamboo sawdust, cassava stem and Chinese fir sawdust | Streptomyces ipomoeae | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 44000, SDS-PAGE | Streptomyces ipomoeae |
Synonyms | Comment | Organism |
---|---|---|
SipoEnXyn10A | - |
Streptomyces ipomoeae |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
75 | 80 | - |
Streptomyces ipomoeae |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
65 | 85 | 65°C: about 45% of maximal activity, 85°C: about 50% of maximal activity | Streptomyces ipomoeae |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
65 | - |
1 h, no loss of activity | Streptomyces ipomoeae |
70 | - |
1 h, 15% loss of activity | Streptomyces ipomoeae |
75 | - |
t1/2: 30 min | Streptomyces ipomoeae |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.5 | - |
- |
Streptomyces ipomoeae |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5 | 9 | pH 5.0: about 50% of maximal activity, pH 9.0: about 55% of maximal activity | Streptomyces ipomoeae |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
4 | 11 | stable | Streptomyces ipomoeae |