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Literature summary for 3.2.1.8 extracted from

  • Xian, L.; Li, Z.; Tang, A.X.; Qin, Y.M.; Li, Q.Y.; Liu, H.B.; Liu, Y.Y.
    A novel neutral and thermophilic endoxylanase from Streptomyces ipomoeae efficiently produced xylobiose from agricultural and forestry residues (2019), Biores. Technol., 285, 121293 .
    View publication on PubMed

Application

Application Comment Organism
food industry the ability of the enzym to produce xylobiose from agricultural and forestry residues proves that it is an excellent candidate enzyme in prebiotic and alternative sweetener industries Streptomyces ipomoeae

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Streptomyces ipomoeae

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
beech wood xylan Km-value: 1.14 g/l, pH 6.5, 75°C Streptomyces ipomoeae

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
44000
-
SDS-PAGE Streptomyces ipomoeae

Organism

Organism UniProt Comment Textmining
Streptomyces ipomoeae MK331807
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Streptomyces ipomoeae

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
197.75
-
substrate: beech wood xylan, pH 6.5, 75°C Streptomyces ipomoeae
601.32
-
substrate: wheat arabinoxylan, pH 6.5, 75°C Streptomyces ipomoeae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
beech wood xylan + H2O hydrolysis of beechwood xylan in endo-action mode releasing xylobiose as its main end product Streptomyces ipomoeae xylobiose + ?
-
?
additional information the xylobiose yield from 10 g corncob and moso bamboo reached 1.123 g and 0.229 g, respectively, at pH 6.5 and 70 °C. High ratios of xylobiose in the xylose-based product of about 85% are obtained from corncob, moso bamboo sawdust, cassava stem and Chinese fir sawdust Streptomyces ipomoeae ?
-
?

Subunits

Subunits Comment Organism
? x * 44000, SDS-PAGE Streptomyces ipomoeae

Synonyms

Synonyms Comment Organism
SipoEnXyn10A
-
Streptomyces ipomoeae

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
75 80
-
Streptomyces ipomoeae

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
65 85 65°C: about 45% of maximal activity, 85°C: about 50% of maximal activity Streptomyces ipomoeae

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
65
-
1 h, no loss of activity Streptomyces ipomoeae
70
-
1 h, 15% loss of activity Streptomyces ipomoeae
75
-
t1/2: 30 min Streptomyces ipomoeae

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.5
-
-
Streptomyces ipomoeae

pH Range

pH Minimum pH Maximum Comment Organism
5 9 pH 5.0: about 50% of maximal activity, pH 9.0: about 55% of maximal activity Streptomyces ipomoeae

pH Stability

pH Stability pH Stability Maximum Comment Organism
4 11 stable Streptomyces ipomoeae