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Literature summary for 3.2.1.8 extracted from
- Penicillium purpurogenum produces a set of endoxylanases identification, heterologous expression, and characterization of a fourth xylanase, XynD, a novel enzyme belonging to glycoside hydrolase family 10 (2019), Appl. Biochem. Biotechnol., 187, 298-309 .
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| gene xynD, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis, recombinant expression in Pichia pastoris strain GS115, the enzyme is secreted | Talaromyces purpureogenus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Talaromyces purpureogenus | A0A2H4RAC5 | i.e. Penicillium purpurogenum | - |
| Talaromyces purpureogenus MYA-38 | A0A2H4RAC5 | i.e. Penicillium purpurogenum | - |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| glycoprotein | the recombinant enzyme may be glycosylated | Talaromyces purpureogenus |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant extracellular enzyme from Pichia pastoris strain GS115 by ultrafiltration, anion exchange chromatography, and again ultrafiltration | Talaromyces purpureogenus |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 47.6 | - |
recombinant enzyme from concentrated cell culture supernatant, pH 7.0, 60°C | Talaromyces purpureogenus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| beechwood xylan + H2O | best substrate | Talaromyces purpureogenus | xylobiose + ? | - |
? |  |
| beechwood xylan + H2O | best substrate | Talaromyces purpureogenus MYA-38 | xylobiose + ? | - |
? | |
| birchwood xylan + H2O | - |
Talaromyces purpureogenus | xylobiose + ? | - |
? | |
| additional information | the enzyme acts as an endoxylanase. The enzyme is also active on xylooligosaccharides, main product is xylobiose and very little xylose is produced. No transglycosylation occurs | Talaromyces purpureogenus | ? | - |
? | |
| additional information | the enzyme acts as an endoxylanase. The enzyme is also active on xylooligosaccharides, main product is xylobiose and very little xylose is produced. No transglycosylation occurs | Talaromyces purpureogenus MYA-38 | ? | - |
? | |
| oat spelt xylan + H2O | - |
Talaromyces purpureogenus | xylobiose + ? | - |
? | |
| wheat arabinoxylan + H2O | best substrate | Talaromyces purpureogenus | xylobiose | - |
? | |
| xylobiose + H2O | low activity | Talaromyces purpureogenus | 2 D-xylose | - |
? | |
| xylobiose + H2O | low activity | Talaromyces purpureogenus MYA-38 | 2 D-xylose | - |
? | |
| xylopentaose + H2O | - |
Talaromyces purpureogenus | xylobiose + xylose | - |
? | |
| xylotetraose + H2O | - |
Talaromyces purpureogenus | 2 xylobiose | - |
? | |
| xylotetraose + H2O | - |
Talaromyces purpureogenus MYA-38 | 2 xylobiose | - |
? | |
| xylotriose + H2O | - |
Talaromyces purpureogenus | xylobiose + xylose | - |
? | |
| xylotriose + H2O | - |
Talaromyces purpureogenus MYA-38 | xylobiose + xylose | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 40997, sequence calculation, x * 60000, recombinant enzyme, SDS-PAGE | Talaromyces purpureogenus |
| More | the enzyme XynD consists of 387 amino acid residues with an N-terminal catalytic module, a linker rich in Ser and Thr residues, and a C-terminal family 1 carbohydrate-binding module | Talaromyces purpureogenus |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| beta-xylanase | UniProt | Talaromyces purpureogenus |
| endoxylanase | - |
Talaromyces purpureogenus |
| XynD | - |
Talaromyces purpureogenus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 60 | - |
recombinant enzyme | Talaromyces purpureogenus |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 40 | 90 | recombinant enzyme, over 80% of maximal activity within this range | Talaromyces purpureogenus |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 40 | - |
purified recombinant enzyme, pH 6.5, 24 h, completely stable | Talaromyces purpureogenus |
| 60 | - |
purified recombinant enzyme, pH 6.5, 60 min, stable | Talaromyces purpureogenus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6.5 | 8.5 | recombinant enzyme | Talaromyces purpureogenus |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 7 | 9 | recombinant enzyme, over 70% of maximal activity within this range | Talaromyces purpureogenus |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 6 | 8 | purified recombinant enzyme, 37°C, 24 h, 80% activity remaining | Talaromyces purpureogenus |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Talaromyces purpureogenus | sequence calculation | - |
5.64 |
General Information
| General Information | Comment | Organism |
|---|---|---|
| evolution | enzyme XynD belongs to the glycoside hydrolase family 10, GH10 | Talaromyces purpureogenus |
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