Cloned (Comment) | Organism |
---|---|
gene xynD, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis, recombinant expression in Pichia pastoris strain GS115, the enzyme is secreted | Talaromyces purpureogenus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Talaromyces purpureogenus | A0A2H4RAC5 | i.e. Penicillium purpurogenum | - |
Talaromyces purpureogenus MYA-38 | A0A2H4RAC5 | i.e. Penicillium purpurogenum | - |
Posttranslational Modification | Comment | Organism |
---|---|---|
glycoprotein | the recombinant enzyme may be glycosylated | Talaromyces purpureogenus |
Purification (Comment) | Organism |
---|---|
recombinant extracellular enzyme from Pichia pastoris strain GS115 by ultrafiltration, anion exchange chromatography, and again ultrafiltration | Talaromyces purpureogenus |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
47.6 | - |
recombinant enzyme from concentrated cell culture supernatant, pH 7.0, 60°C | Talaromyces purpureogenus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
beechwood xylan + H2O | best substrate | Talaromyces purpureogenus | xylobiose + ? | - |
? | |
beechwood xylan + H2O | best substrate | Talaromyces purpureogenus MYA-38 | xylobiose + ? | - |
? | |
birchwood xylan + H2O | - |
Talaromyces purpureogenus | xylobiose + ? | - |
? | |
additional information | the enzyme acts as an endoxylanase. The enzyme is also active on xylooligosaccharides, main product is xylobiose and very little xylose is produced. No transglycosylation occurs | Talaromyces purpureogenus | ? | - |
? | |
additional information | the enzyme acts as an endoxylanase. The enzyme is also active on xylooligosaccharides, main product is xylobiose and very little xylose is produced. No transglycosylation occurs | Talaromyces purpureogenus MYA-38 | ? | - |
? | |
oat spelt xylan + H2O | - |
Talaromyces purpureogenus | xylobiose + ? | - |
? | |
wheat arabinoxylan + H2O | best substrate | Talaromyces purpureogenus | xylobiose | - |
? | |
xylobiose + H2O | low activity | Talaromyces purpureogenus | 2 D-xylose | - |
? | |
xylobiose + H2O | low activity | Talaromyces purpureogenus MYA-38 | 2 D-xylose | - |
? | |
xylopentaose + H2O | - |
Talaromyces purpureogenus | xylobiose + xylose | - |
? | |
xylotetraose + H2O | - |
Talaromyces purpureogenus | 2 xylobiose | - |
? | |
xylotetraose + H2O | - |
Talaromyces purpureogenus MYA-38 | 2 xylobiose | - |
? | |
xylotriose + H2O | - |
Talaromyces purpureogenus | xylobiose + xylose | - |
? | |
xylotriose + H2O | - |
Talaromyces purpureogenus MYA-38 | xylobiose + xylose | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 40997, sequence calculation, x * 60000, recombinant enzyme, SDS-PAGE | Talaromyces purpureogenus |
More | the enzyme XynD consists of 387 amino acid residues with an N-terminal catalytic module, a linker rich in Ser and Thr residues, and a C-terminal family 1 carbohydrate-binding module | Talaromyces purpureogenus |
Synonyms | Comment | Organism |
---|---|---|
beta-xylanase | UniProt | Talaromyces purpureogenus |
endoxylanase | - |
Talaromyces purpureogenus |
XynD | - |
Talaromyces purpureogenus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
recombinant enzyme | Talaromyces purpureogenus |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | 90 | recombinant enzyme, over 80% of maximal activity within this range | Talaromyces purpureogenus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
purified recombinant enzyme, pH 6.5, 24 h, completely stable | Talaromyces purpureogenus |
60 | - |
purified recombinant enzyme, pH 6.5, 60 min, stable | Talaromyces purpureogenus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.5 | 8.5 | recombinant enzyme | Talaromyces purpureogenus |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
7 | 9 | recombinant enzyme, over 70% of maximal activity within this range | Talaromyces purpureogenus |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
6 | 8 | purified recombinant enzyme, 37°C, 24 h, 80% activity remaining | Talaromyces purpureogenus |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Talaromyces purpureogenus | sequence calculation | - |
5.64 |
General Information | Comment | Organism |
---|---|---|
evolution | enzyme XynD belongs to the glycoside hydrolase family 10, GH10 | Talaromyces purpureogenus |