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Literature summary for 3.2.1.8 extracted from

  • Boonyapakron, K.; Jaruwat, A.; Liwnaree, B.; Nimchua, T.; Champreda, V.; Chitnumsub, P.
    Structure-based protein engineering for thermostable and alkaliphilic enhancement of endo-beta-1,4-xylanase for applications in pulp bleaching (2017), J. Biotechnol., 259, 95-102 .
    View publication on PubMed

Crystallization (Commentary)

Crystallization (Comment) Organism
molecular modeling of structure unidentified microorganism

Protein Variants

Protein Variants Comment Organism
E142H complete loss of activity unidentified microorganism
E244A 1% of wild-type activity unidentified microorganism
E244H complete loss of activity unidentified microorganism
K231R/K223R/K227R 145% of wild-type activity unidentified microorganism
K73R/K185R 133% of wild-type activity unidentified microorganism
K73R/K185R/K231R/K223R/K227R 146% of wild-type activity unidentified microorganism
T28C/T60C 171% of wild-type activity, 2-3fold increases in bagasse hydrolysis at pH 9.0 and 60°C compared to the wild-type unidentified microorganism
T28C/T60C/T48F/L59F 134% of wild-type activity unidentified microorganism
T28C/T60C/T77C/E249C 158% of wild-type activity, 2-3fold increases in bagasse hydrolysis at pH 9.0 and 60°C compared to the wild-type unidentified microorganism
V5N/V6N/K7R/K223R/K227R 130% of wild-type activity unidentified microorganism
V5N/V6N/K7R/K223R/K227R/T28C/T60C 154% of wild-type activity unidentified microorganism

Organism

Organism UniProt Comment Textmining
unidentified microorganism
-
derived from the termite gut symbiont metagenome
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
beechwood xylan + H2O
-
unidentified microorganism ?
-
?

Synonyms

Synonyms Comment Organism
Xyn12.2
-
unidentified microorganism

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1210
-
beechwood xylan wild-type, pH 8.0, 50°C unidentified microorganism