Activating Compound | Comment | Organism | Structure |
---|---|---|---|
Triton X-100 | 0.5%, about 120% of initial activity | Luteimicrobium xylanilyticum | |
Tween 80 | 0.5%, about 120% of initial activity | Luteimicrobium xylanilyticum |
Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Luteimicrobium xylanilyticum |
Protein Variants | Comment | Organism |
---|---|---|
additional information | construction of a deletion mutant lacking the C-terminal ricin-type beta-trefoil lectin domain-like part. Mutant shows a decrease in binding capacities to lignin and insoluble polysaccharides | Luteimicrobium xylanilyticum |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Hg2+ | 1 mM, complete loss of activity | Luteimicrobium xylanilyticum | |
additional information | stimulation or inhibition by 1 mM of Ca2+,Ni2+, Zn2+, Mg2+, Mn2+, Sn2+, Ba2+, Co2+, and Fe2+ is marginal | Luteimicrobium xylanilyticum | |
N-bromosuccinimide | 5 mM, complete loss of activity | Luteimicrobium xylanilyticum |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
oat spelt xylan | Km value 1.3 mg/ml, pH 6.0, 65°C | Luteimicrobium xylanilyticum | |
additional information | - |
beechwood xylan | Km value 1.4 mg/ml, pH 6.0, 65°C | Luteimicrobium xylanilyticum | |
additional information | - |
birchwood xylan | Km value 1.5 mg/ml, pH 6.0, 65°C | Luteimicrobium xylanilyticum | |
additional information | - |
wheat arabinoxylan | Km value 2.8 mg/ml, pH 6.0, 65°C | Luteimicrobium xylanilyticum |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
additional information | stimulation or inhibition by 1 mM of Ca2+,Ni2+, Zn2+, Mg2+, Mn2+, Sn2+, Ba2+, Co2+, and Fe2+ is marginal | Luteimicrobium xylanilyticum |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Luteimicrobium xylanilyticum | A0A286LNP2 | - |
- |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
74.8 | - |
substrate oat spelt xylan, C-terminal truncated mutant, pH 6.0, 65°C | Luteimicrobium xylanilyticum |
254 | - |
substrate oat spelt xylan, wild-type, pH 6.0, 65°C | Luteimicrobium xylanilyticum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
beechwood xylan + H2O | - |
Luteimicrobium xylanilyticum | ? | - |
? | |
birchwood xylan + H2O | - |
Luteimicrobium xylanilyticum | xylotetraose + xylotriose + xylobiose + xylose | hydrolysis product composition is 1.4% xylose, 37.1% xylobiose, 58.9% xylotriose and 2.6% xylotetraose | ? | |
additional information | activity decreases in the following order: oat spelt xylan, beechwood xylan, birchwood xylan, wheat arabinoxylan. No substrates: xyloglucan, carboxymethylcellulose, locust beangum, and 4-nitrophenyl sugar derivatives | Luteimicrobium xylanilyticum | ? | - |
? | |
oat spelt xylan + H2O | - |
Luteimicrobium xylanilyticum | ? | - |
? | |
wheat arabinoxylan + H2O | - |
Luteimicrobium xylanilyticum | ? | - |
? | |
xylohexaose + H2O | - |
Luteimicrobium xylanilyticum | xylopentaose + xylotetraose + xylotriose + xylobiose + xylose | hydrolysis product composition is 1.2% xylose, 29.8% xylobiose, 56.4% xylotriose, 10.9% xylotetraose and 1.7% xylopentaose | ? | |
xylopentaose + H2O | - |
Luteimicrobium xylanilyticum | xylopentaose + xylotetraose + xylotriose + xylobiose + xylose | hydrolysis product composition is 1.2% xylose, 38.4% xylobiose, 49.1% xylotriose, 6.7% xylotetraose and 4.6% xylopentaose | ? | |
xylotetraose + H2O | - |
Luteimicrobium xylanilyticum | xylotetraose + xylotriose + xylobiose + xylose | hydrolysis product composition is 1.9% xylose, 34.9% xylobiose, 49.2% xylotriose and 14.0% xylotetraose | ? |
Subunits | Comment | Organism |
---|---|---|
? | x * 49000, SDS-PAGE, recombinant protein, x * 50480, calculated | Luteimicrobium xylanilyticum |
Synonyms | Comment | Organism |
---|---|---|
xylM | - |
Luteimicrobium xylanilyticum |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
45 | - |
C-terminal truncated mutant | Luteimicrobium xylanilyticum |
65 | - |
wild-type | Luteimicrobium xylanilyticum |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
C-terminal truncated mutant, loss of activity above 40°C | Luteimicrobium xylanilyticum |
60 | - |
1 h, 80% residual activity | Luteimicrobium xylanilyticum |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
85 | - |
wheat arabinoxylan | pH 6.0, 65°C | Luteimicrobium xylanilyticum | |
222 | - |
birchwood xylan | pH 6.0, 65°C | Luteimicrobium xylanilyticum | |
248 | - |
beechwood xylan | pH 6.0, 65°C | Luteimicrobium xylanilyticum | |
390 | - |
oat spelt xylan | pH 6.0, 65°C | Luteimicrobium xylanilyticum |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5 | - |
C-terminal truncated mutant | Luteimicrobium xylanilyticum |
6 | - |
wild-type | Luteimicrobium xylanilyticum |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
birchwood xylan | kcat/Km value 153 ml/mg/s, pH 6.0, 65°C | Luteimicrobium xylanilyticum | |
additional information | - |
beechwood xylan | kcat/Km value 178 ml/mg/s, pH 6.0, 65°C | Luteimicrobium xylanilyticum | |
additional information | - |
wheat arabinoxylan | kcat/Km value 30 ml/mg/s, pH 6.0, 65°C | Luteimicrobium xylanilyticum | |
additional information | - |
oat spelt xylan | kcat/Km value 309 ml/mg/s, pH 6.0, 65°C | Luteimicrobium xylanilyticum |