Protein Variants | Comment | Organism |
---|---|---|
additional information | random mutagenesis leads to mutant M7 with improved kinetic and thermodynamic properties | Thermomyces lanuginosus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
oat spelt xylan | Km value of wild-type 0.112 mg/ml, of mutant M7 0.098 mg/ml, respectively, pH 6.0, 50°C | Thermomyces lanuginosus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
24000 | - |
gel filtration | Thermomyces lanuginosus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Thermomyces lanuginosus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Thermomyces lanuginosus |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
15501 | - |
wild-type, pH 6.0, 50°C | Thermomyces lanuginosus |
17778 | - |
mutant M7, pH 6.0, 50°C | Thermomyces lanuginosus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
oat spelt xylan + H2O | - |
Thermomyces lanuginosus | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 24000, SDS-PAGE | Thermomyces lanuginosus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
65 | - |
wild-type | Thermomyces lanuginosus |
70 | - |
mutant M7 | Thermomyces lanuginosus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
5967 | - |
oat spelt xylan | wild-type, pH 6.0, 50°C | Thermomyces lanuginosus | |
7159 | - |
oat spelt xylan | mutant M7, pH 6.0, 50°C | Thermomyces lanuginosus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.3 | - |
wild-type | Thermomyces lanuginosus |
8.1 | - |
mutant M7 | Thermomyces lanuginosus |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
oat spelt xylan | kcat/Km value of wild-type 53279 ml/mg/s, of mutant M7 73048 ml/mg/s, respectively, pH 6.0, 50°C | Thermomyces lanuginosus |