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Literature summary for 3.2.1.8 extracted from
- Characterization of a highly thermostable glycoside hydrolase family 10 xylanase from Malbranchea cinnamomea (2014), Int. J. Biol. Macromol., 70, 482-489 .
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
oat spelt xylan | Km value 1.5 mg/ml, pH 6.5, 50°C | Malbranchea cinnamomea | |
| additional information | - |
beechwood xylan | Km value 3.0 mg/ml, pH 6.5, 50°C | Malbranchea cinnamomea | |
| additional information | - |
birchwood xylan | Km value 7.1 mg/ml, pH 6.5, 50°C | Malbranchea cinnamomea |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| extracellular | - |
Malbranchea cinnamomea | - |
- |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 40500 | - |
gel filtration | Malbranchea cinnamomea |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Malbranchea cinnamomea | A0A088BC14 | - |
- |
| Malbranchea cinnamomea S168 | A0A088BC14 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Malbranchea cinnamomea |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 878 | - |
substrate birchwood xylan, pH 6.5, 50°C | Malbranchea cinnamomea |
| 1028 | - |
substrate beechwood xylan, pH 6.5, 50°C | Malbranchea cinnamomea |
| 1478 | - |
substrate oat spelt xylan, pH 6.5, 50°C | Malbranchea cinnamomea |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| beechwood xylan + H2O | - |
Malbranchea cinnamomea | ? | - |
? |  |
| beechwood xylan + H2O | - |
Malbranchea cinnamomea S168 | ? | - |
? | |
| birchwood xylan + H2O | - |
Malbranchea cinnamomea | ? | - |
? | |
| birchwood xylan + H2O | - |
Malbranchea cinnamomea S168 | ? | - |
? | |
| additional information | enzyme exhibits strict specificity for various xylans as substrates, but displays no activity toward avicel, carboxymethyl cellulose, locust bean gum and lichenan, or 4-nitrophenyl beta-D-glucopyranoside, 4-nitrophenyl beta-D-fucopyranoside and 4-nitrophenyl beta-D-galactopyranoside | Malbranchea cinnamomea | ? | - |
? | |
| additional information | enzyme exhibits strict specificity for various xylans as substrates, but displays no activity toward avicel, carboxymethyl cellulose, locust bean gum and lichenan, or 4-nitrophenyl beta-D-glucopyranoside, 4-nitrophenyl beta-D-fucopyranoside and 4-nitrophenyl beta-D-galactopyranoside | Malbranchea cinnamomea S168 | ? | - |
? | |
| oat spelt xylan + H2O | - |
Malbranchea cinnamomea | ? | - |
? | |
| oat spelt xylan + H2O | - |
Malbranchea cinnamomea S168 | ? | - |
? | |
| xylotetraose + H2O | - |
Malbranchea cinnamomea | 2 xylobiose | - |
? | |
| xylotetraose + H2O | - |
Malbranchea cinnamomea S168 | 2 xylobiose | - |
? | |
| xylotriose + H2O | - |
Malbranchea cinnamomea | xylobiose + ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 43500, SDS-PAGE | Malbranchea cinnamomea |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| Xyn10 | - |
Malbranchea cinnamomea |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 80 | - |
- |
Malbranchea cinnamomea |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 70 | - |
half-life 76 min | Malbranchea cinnamomea |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 4.18 | - |
beechwood xylan | pH 6.5, 50°C | Malbranchea cinnamomea | |
| 4.26 | - |
birchwood xylan | pH 6.5, 50°C | Malbranchea cinnamomea | |
| 4.58 | - |
oat spelt xylan | pH 6.5, 50°C | Malbranchea cinnamomea |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6.5 | - |
- |
Malbranchea cinnamomea |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 4 | 10.5 | - |
Malbranchea cinnamomea |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Malbranchea cinnamomea | calculated | - |
4.4 |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
birchwood xylan | kcat/Km value 0.6 ml/mg/s, pH 6.5, 50°C | Malbranchea cinnamomea | |
| additional information | - |
beechwood xylan | kcat/Km value 1.39 ml/mg/s, pH 6.5, 50°C | Malbranchea cinnamomea | |
| additional information | - |
oat spelt xylan | kcat/Km value 3.06 ml/mg/s, pH 6.5, 50°C | Malbranchea cinnamomea |
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