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Literature summary for 3.2.1.8 extracted from

  • Prabhu, K. A.; R. Maheshwari
    : Biochemical properties of xylanases from a thermophilic fungus, Melanocarpus albomyces, and their action on plant cell walls (1999), J. Biosci., 24, 461-470.
No PubMed abstract available

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
xylan Km for larchwood xylan 0.33 mg/ml, xylanase IA. Km for larchwood xylan 1.69 mg/ml, xylanase IIIA Melanocarpus albomyces
additional information
-
xylan Km for xylanase IA: 0.33 mg/ml, Km for xylanase IIIA: 1.69 mg/ml Melanocarpus albomyces

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
24000
-
xylanase IIIA Melanocarpus albomyces
38000
-
xylanase IA Melanocarpus albomyces

Organism

Organism UniProt Comment Textmining
Melanocarpus albomyces
-
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein xlanase IA contains 7% carbohydrate. Xylanase IIIA contains no detectable carbohydrate Melanocarpus albomyces
glycoprotein xylanase IA contains 7% carbohydrate, xylanase III contains detectable carbohydrate Melanocarpus albomyces

Purification (Commentary)

Purification (Comment) Organism
two xylanase activities, a minor xylanase IA and a major xylanase IIIA, are purified Melanocarpus albomyces
two xylanase activities, a minor xylanase IA and a major xylanase IIIA, were purified to apparent homogeneity from bagasse-grown cultures Melanocarpus albomyces

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
xylan + H2O xylanase IA and xylanase IIIA are bothe specific for beta-1,4 xylose-rich polymer Melanocarpus albomyces xylobiose + xylotriose + xylooligosaccharides
-
?
xylan + H2O
-
Melanocarpus albomyces xylotriose + xylotriose + ?
-
?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
65
-
-
Melanocarpus albomyces

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
50
-
stable Melanocarpus albomyces

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.6
-
xylanase IIIA Melanocarpus albomyces
6.6
-
xylanase IA Melanocarpus albomyces

pH Stability

pH Stability pH Stability Maximum Comment Organism
5 10 50°C, 24 h, stable, xylanase IA and xylanase IIIA Melanocarpus albomyces
5 10 50°C, 24, stable Melanocarpus albomyces

Expression

Organism Comment Expression
Melanocarpus albomyces activity is inducible not only by hemicellulosic material but also by the monomeric pentosan unit of xylan but not by glucose up
Melanocarpus albomyces xylanase activity is inducible not only by hemicellulosic material but also by the monomeric pentosan unit of xylan but not by glucose up