KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
xylan | Km for larchwood xylan 0.33 mg/ml, xylanase IA. Km for larchwood xylan 1.69 mg/ml, xylanase IIIA | Melanocarpus albomyces | |
additional information | - |
xylan | Km for xylanase IA: 0.33 mg/ml, Km for xylanase IIIA: 1.69 mg/ml | Melanocarpus albomyces |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
24000 | - |
xylanase IIIA | Melanocarpus albomyces |
38000 | - |
xylanase IA | Melanocarpus albomyces |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Melanocarpus albomyces | - |
- |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
glycoprotein | xlanase IA contains 7% carbohydrate. Xylanase IIIA contains no detectable carbohydrate | Melanocarpus albomyces |
glycoprotein | xylanase IA contains 7% carbohydrate, xylanase III contains detectable carbohydrate | Melanocarpus albomyces |
Purification (Comment) | Organism |
---|---|
two xylanase activities, a minor xylanase IA and a major xylanase IIIA, are purified | Melanocarpus albomyces |
two xylanase activities, a minor xylanase IA and a major xylanase IIIA, were purified to apparent homogeneity from bagasse-grown cultures | Melanocarpus albomyces |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
xylan + H2O | xylanase IA and xylanase IIIA are bothe specific for beta-1,4 xylose-rich polymer | Melanocarpus albomyces | xylobiose + xylotriose + xylooligosaccharides | - |
? | |
xylan + H2O | - |
Melanocarpus albomyces | xylotriose + xylotriose + ? | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
65 | - |
- |
Melanocarpus albomyces |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
stable | Melanocarpus albomyces |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5.6 | - |
xylanase IIIA | Melanocarpus albomyces |
6.6 | - |
xylanase IA | Melanocarpus albomyces |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
5 | 10 | 50°C, 24 h, stable, xylanase IA and xylanase IIIA | Melanocarpus albomyces |
5 | 10 | 50°C, 24, stable | Melanocarpus albomyces |
Organism | Comment | Expression |
---|---|---|
Melanocarpus albomyces | activity is inducible not only by hemicellulosic material but also by the monomeric pentosan unit of xylan but not by glucose | up |
Melanocarpus albomyces | xylanase activity is inducible not only by hemicellulosic material but also by the monomeric pentosan unit of xylan but not by glucose | up |