Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Thermoascus aurantiacus |
Protein Variants | Comment | Organism |
---|---|---|
H209N | mutant shows increased thermostability relative to the wild type at 70°C and 75°C and is stable in the pH range 8.0-10.0, similar to wild-type | Thermoascus aurantiacus |
N257D | mutant shows increased thermostability relative to the wild type at 70°C and 75°C and is stable in the pH range 5.0-10.0 | Thermoascus aurantiacus |
Q158R | mutant shows increased thermostability relative to the wild type at 70°C and 75°C and is stable in the pH range 5.0-10.0, | Thermoascus aurantiacus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Thermoascus aurantiacus | - |
- |
- |
Thermoascus aurantiacus CBMAI 756 | - |
- |
- |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
550 | - |
mutant N257D, pH 5.5, 70°C | Thermoascus aurantiacus |
763 | - |
mutant Q158R, pH 4.5, 65°C | Thermoascus aurantiacus |
1284 | - |
wild-type, pH 5, 65°C | Thermoascus aurantiacus |
1849 | - |
mutant H209N, pH 5.0, 65°C | Thermoascus aurantiacus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
beechwood xylan + H2O | - |
Thermoascus aurantiacus | xylo-oligosaccharides + ? | - |
? | |
beechwood xylan + H2O | - |
Thermoascus aurantiacus CBMAI 756 | xylo-oligosaccharides + ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
XynA | - |
Thermoascus aurantiacus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
65 | - |
wild-type and mutants Q158A, H209N | Thermoascus aurantiacus |
70 | - |
mutant N257D | Thermoascus aurantiacus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
70.4 | - |
melting temperature, mutant N257D | Thermoascus aurantiacus |
70.9 | - |
melting temperature, wild-type and mutant Q158R | Thermoascus aurantiacus |
71.3 | - |
melting temperature, mutant H209N | Thermoascus aurantiacus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
4.5 | - |
mutant Q158A | Thermoascus aurantiacus |
5 | - |
wild-type and mutant H209N | Thermoascus aurantiacus |
5.5 | - |
mutant N257D | Thermoascus aurantiacus |