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Literature summary for 3.2.1.8 extracted from

  • de Souza, A.R.; de Araujo, G.C.; Zanphorlin, L.M.; Ruller, R.; Franco, F.C.; Torres, F.A.; Mertens, J.A.; Bowman, M.J.; Gomes, E.; Da Silva, R.
    Engineering increased thermostability in the GH-10 endo-1,4-beta-xylanase from Thermoascus aurantiacus CBMAI 756 (2016), Int. J. Biol. Macromol., 93, 20-26.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Thermoascus aurantiacus

Protein Variants

Protein Variants Comment Organism
H209N mutant shows increased thermostability relative to the wild type at 70°C and 75°C and is stable in the pH range 8.0-10.0, similar to wild-type Thermoascus aurantiacus
N257D mutant shows increased thermostability relative to the wild type at 70°C and 75°C and is stable in the pH range 5.0-10.0 Thermoascus aurantiacus
Q158R mutant shows increased thermostability relative to the wild type at 70°C and 75°C and is stable in the pH range 5.0-10.0, Thermoascus aurantiacus

Organism

Organism UniProt Comment Textmining
Thermoascus aurantiacus
-
-
-
Thermoascus aurantiacus CBMAI 756
-
-
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
550
-
mutant N257D, pH 5.5, 70°C Thermoascus aurantiacus
763
-
mutant Q158R, pH 4.5, 65°C Thermoascus aurantiacus
1284
-
wild-type, pH 5, 65°C Thermoascus aurantiacus
1849
-
mutant H209N, pH 5.0, 65°C Thermoascus aurantiacus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
beechwood xylan + H2O
-
Thermoascus aurantiacus xylo-oligosaccharides + ?
-
?
beechwood xylan + H2O
-
Thermoascus aurantiacus CBMAI 756 xylo-oligosaccharides + ?
-
?

Synonyms

Synonyms Comment Organism
XynA
-
Thermoascus aurantiacus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
65
-
wild-type and mutants Q158A, H209N Thermoascus aurantiacus
70
-
mutant N257D Thermoascus aurantiacus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
70.4
-
melting temperature, mutant N257D Thermoascus aurantiacus
70.9
-
melting temperature, wild-type and mutant Q158R Thermoascus aurantiacus
71.3
-
melting temperature, mutant H209N Thermoascus aurantiacus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
4.5
-
mutant Q158A Thermoascus aurantiacus
5
-
wild-type and mutant H209N Thermoascus aurantiacus
5.5
-
mutant N257D Thermoascus aurantiacus