Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.2.1.8 extracted from

  • Garcia-Huante, Y.; Cayetano-Cruz, M.; Santiago-Hernandez, A.; Cano-Ramirez, C.; Marsch-Moreno, R.; Campos, J.E.; Aguilar-Osorio, G.; Benitez-Cardoza, C.G.; Trejo-Estrada, S.; Hidalgo-Lara, M.E.
    The thermophilic biomass-degrading fungus Thielavia terrestris Co3Bag1 produces a hyperthermophilic and thermostable beta-1,4-xylanase with exo- and endo-activity (2016), Extremophiles, 21, 175-186.
    View publication on PubMed
Search for more literature for 3.2.1.8

Inhibitors

Inhibitors Comment Organism Structure
Cu2+ 1 mM, 47% resiudal activity Thermothielavioides terrestris
Hg2+ 1 mM, 55% resiudal activity Thermothielavioides terrestris
Mg2+ 1 mM, 69% resiudal activity Thermothielavioides terrestris
Mn2+ 1 mM, 57% resiudal activity Thermothielavioides terrestris
Ni2+ 1 mM, 77% resiudal activity Thermothielavioides terrestris

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 beechwood xylan Km value 0.41 mg/ml, pH 5.5, 85°C Thermothielavioides terrestris
additional information
-
 birchwood xylan Km value 0.55 mg/ml, pH 5.5, 85°C Thermothielavioides terrestris
additional information
-
 oat spelt xylan Km value 0.58 mg/ml, pH 5.5, 85°C Thermothielavioides terrestris
additional information
-
 carboxymethyl cellulose Km value 6.6 mg/ml, pH 5.5, 85°C Thermothielavioides terrestris

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular
-
 Thermothielavioides terrestris
-
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
82000
-
 x * 82000, SDS-PAGE Thermothielavioides terrestris

Organism

Organism UniProt Comment Textmining
Thermothielavioides terrestris
-
-
-
Thermothielavioides terrestris Co3Bag1
-
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein about 20% of total mass are glycosyl residues Thermothielavioides terrestris

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
169
-
 pH 5.5, 85°C Thermothielavioides terrestris

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
beechwood xylan + H2O
-
 Thermothielavioides terrestris xylobiose + ? products are xylobiose, an unknown oligosaccharide containing about five residues of xylose and a small amount of xylose ?
beechwood xylan + H2O
-
 Thermothielavioides terrestris Co3Bag1 xylobiose + ? products are xylobiose, an unknown oligosaccharide containing about five residues of xylose and a small amount of xylose ?
birchwood xylan + H2O
-
 Thermothielavioides terrestris ?
-
 ?
birchwood xylan + H2O
-
 Thermothielavioides terrestris Co3Bag1 ?
-
 ?
carboxymethyl cellulose + H2O
-
 Thermothielavioides terrestris ?
-
 ?
carboxymethyl cellulose + H2O
-
 Thermothielavioides terrestris Co3Bag1 ?
-
 ?
additional information no substrate: avicel Thermothielavioides terrestris ?
-
 ?
additional information no substrate: avicel Thermothielavioides terrestris Co3Bag1 ?
-
 ?
oat spelt xylan + H2O
-
 Thermothielavioides terrestris ?
-
 ?
oat spelt xylan + H2O
-
 Thermothielavioides terrestris Co3Bag1 ?
-
 ?
Remazol Brilliant Blue-Xylan + H2O
-
 Thermothielavioides terrestris ?
-
 ?

Subunits

Subunits Comment Organism
? x * 82000, SDS-PAGE Thermothielavioides terrestris

Synonyms

Synonyms Comment Organism
XynA
-
 Thermothielavioides terrestris

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
85
-
-
 Thermothielavioides terrestris

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
65 80 70-80% of maximum activity Thermothielavioides terrestris

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
65
-
 half-life 23.1 days Thermothielavioides terrestris

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.5
-
-
 Thermothielavioides terrestris

pH Range

pH Minimum pH Maximum Comment Organism
4.5 10 more than 90% of maximum activity Thermothielavioides terrestris

Expression

Organism Comment Expression
Thermothielavioides terrestris higher activity is obtained when Tthe organism is grown on carboxymethyl cellulose (1.6 U/ml) than on xylan (0.7 U/ml), after 96 h of culture at 45°C additional information