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Literature summary for 3.2.1.8 extracted from

  • Yang, W.; Bai, Y.; Yang, P.; Luo, H.; Huang, H.; Meng, K.; Shi, P.; Wang, Y.; Yao, B.
    A novel bifunctional GH51 exo-alpha-L-arabinofuranosidase/endo-xylanase from Alicyclobacillus sp. A4 with significant biomass-degrading capacity (2015), Biotechnol. Biofuels, 8, 197.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.2.1.8

Application

Application Comment Organism
degradation Abf51A shows greater synergistic effect in combination with xylanase (2.92fold) on wheat arabinoxylan degradation than other reported enzymes, the amounts of arabinose, xylose, and xylobiose are all increased in comparison to that by the enzymes acting individually Alicyclobacillus sp.

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Alicyclobacillus sp.

Organism

Organism UniProt Comment Textmining
Alicyclobacillus sp. A0A0P0ISK8 cf. EC 3.2.1.55
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information the enzyme is an arabinofuranose that may also act as xylanosidase. The enzyme additionally releases xylobiose and xylotriose from wheat arabinoxylan and is active on xylooligosaccharides (xylohexaose 1.2/mM/min, xylopentaose 6.9/mM/min, and xylotetraose 19.7/mM/min), however a lower level of activity Alicyclobacillus sp. ?
-
 ?
wheat arabinoxylan + H2O
-
 Alicyclobacillus sp. xylobiose + xylotriose + ?
-
 ?
xylohexaose + H2O
-
 Alicyclobacillus sp. ?
-
 ?
xylopentaose + H2O
-
 Alicyclobacillus sp. ?
-
 ?
xylotetraose + H2O
-
 Alicyclobacillus sp. ?
-
 ?

Synonyms

Synonyms Comment Organism
Abf51A
-
 Alicyclobacillus sp.

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
60
-
-
 Alicyclobacillus sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6
-
-
 Alicyclobacillus sp.

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.02
-
 xylohexaose pH 6.0, 60°C Alicyclobacillus sp.
0.12
-
 xylopentaose pH 6.0, 60°C Alicyclobacillus sp.
0.33
-
 xylotetraose pH 6.0, 60°C Alicyclobacillus sp.