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Literature summary for 3.2.1.8 extracted from

  • Tuohy, M. G.; Puls, J.; Claeyssens, M.; Vrsanska, M.; Coughlan, M.P.
    The xylan-degrading enzyme system of Talaromyces emersonii: novel enzymes with activity against aryl beta-D-xylosides and unsubstituted xylans (1993), Biochem. J., 290, 515-523.
No PubMed abstract available
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KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.4
-
 4-nitrophenyl-4-O-beta-D-xylopyranosyl-beta-D-xylopyranose pH 5, 50°C, xylanase II Rasamsonia emersonii
1.4
-
 4-nitrophenyl xylopyranoside pH 5, 50°C, xylanase III Rasamsonia emersonii
1.8
-
 2-nitrophenyl xylopyranoside pH 5, 50°C, xylanase II Rasamsonia emersonii
3.3
-
 2-nitrophenyl xylopyranoside pH 5, 50°C, xylanase III Rasamsonia emersonii
32.9
-
 4-nitrophenyl xylopyranoside pH 5, 50°C, xylanase II Rasamsonia emersonii

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular
-
 Rasamsonia emersonii
-
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
49800
-
-
 Rasamsonia emersonii
54200
-
 xylanase III, SDS-PAGE Rasamsonia emersonii
131000
-
 xylanase II, gel filtration Rasamsonia emersonii

Organism

Organism UniProt Comment Textmining
Rasamsonia emersonii
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Rasamsonia emersonii

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.2
-
 substrate: 4-nitrophenyl xylopyranoside, pH 5, 50°C, xylanase III Rasamsonia emersonii
0.4
-
 substrate: 4-nitrophenyl-4-O-beta-D-xylopyranosyl-beta-D-xylopyranose, pH 5, 50°C, xylanase III Rasamsonia emersonii
2.9
-
 substrate: 4-nitrophenyl xylopyranoside, pH 5, 50°C, xylanase II Rasamsonia emersonii
92.6
-
 substrate: 2-nitrophenyl xylopyranoside, pH 5, 50°C, xylanase III Rasamsonia emersonii
163.1
-
 substrate: 2-nitrophenyl xylopyranoside, pH 5, 50°C, xylanase II Rasamsonia emersonii
287.5
-
 substrate: 4-nitrophenyl-4-O-beta-D-xylopyranosyl-beta-D-xylopyranose, pH 5, 50°C, xylanase II Rasamsonia emersonii

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-nitrophenyl xylopyranoside + H2O
-
 Rasamsonia emersonii 2-nitrophenol + xylopyranose
-
 ?
4-nitrophenyl xylopyranoside + H2O
-
 Rasamsonia emersonii 4-nitrophenol + xylopyranose
-
 ?
4-nitrophenyl-4-O-beta-D-xylopyranosyl-beta-D-xylopyranose + H2O
-
 Rasamsonia emersonii ?
-
 ?
xylan + H2O lenzing xylan Rasamsonia emersonii xylobiose + xylotriose + ? and small amounts of xylotetraose and xylopentaose ?

Subunits

Subunits Comment Organism
dimer 2 * 49800, xylanase II Rasamsonia emersonii
monomer 1 * 54200, xylanase III Rasamsonia emersonii

Synonyms

Synonyms Comment Organism
xylanase II
-
 Rasamsonia emersonii
xylanase III
-
 Rasamsonia emersonii

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
61
-
 4-nitrophenyl xylopyranoside pH 5, 50°C, xylanase III Rasamsonia emersonii
345
-
 2-nitrophenyl xylopyranoside pH 5, 50°C, xylanase III Rasamsonia emersonii
523
-
 2-nitrophenyl xylopyranoside pH 5, 50°C, xylanase II Rasamsonia emersonii
898
-
 4-nitrophenyl xylopyranoside pH 5, 50°C, xylanase II Rasamsonia emersonii

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
3.5
-
 xylanase III Rasamsonia emersonii
4.2
-
 xylanase II Rasamsonia emersonii

pI Value

Organism Comment pI Value Maximum pI Value
Rasamsonia emersonii xylanase III, isoelectric focusing
-
 4.2
Rasamsonia emersonii xylanase II, isoelectric focusing
-
 5.3

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
27
-
 4-nitrophenyl xylopyranoside pH 5, 50°C, xylanase II Rasamsonia emersonii
43
-
 4-nitrophenyl xylopyranoside pH 5, 50°C, xylanase III Rasamsonia emersonii
104
-
 2-nitrophenyl xylopyranoside pH 5, 50°C, xylanase III Rasamsonia emersonii
292
-
 2-nitrophenyl xylopyranoside pH 5, 50°C, xylanase II Rasamsonia emersonii
7190
-
 4-nitrophenyl-4-O-beta-D-xylopyranosyl-beta-D-xylopyranose pH 5, 50°C, xylanase II Rasamsonia emersonii