Literature summary for 3.2.1.8 extracted from

Wang, Q.; Luo, Y.; He, B.; Jiang, L.S.; Liu, J.X.; Wang, J.K.
Characterization of a novel xylanase gene from rumen content of Hu sheep (2015), Appl. Biochem. Biotechnol., 177, 1424-1436.

Search for more literature for 3.2.1.8

Cloned(Commentary)

Cloned (Comment)	Organism
expression in Escherichia coli	uncultured bacterium

Inhibitors

Inhibitors	Comment	Organism	Structure
Ag+	10 mM, 16% residual activity	uncultured bacterium
EDTA	10 mM, 72% residual activity	uncultured bacterium
SDS	1%, no residual activity	uncultured bacterium

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
additional information	-	birchwood xylan	Km value 4.39 mg/ml, pH 6.0, 50°C	uncultured bacterium

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Mn2+	10 mM, 117% of initial activtiy	uncultured bacterium

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
71300	-	-	uncultured bacterium
80000	-	-	uncultured bacterium

Organism

Organism	UniProt	Comment	Textmining
uncultured bacterium	-	isolated from the rumen contents of Hu sheep	-

Source Tissue

Source Tissue	Comment	Organism	Textmining

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
664.7	-	pH 6.0, 50°C	uncultured bacterium

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
birchwood xylan + H2O	-	uncultured bacterium	xylobiose + xylose + ?	xylobiose, 74% of products, plus 21% xylose	?

Subunits

Subunits	Comment	Organism
?	x * 71300, calculated, x * 80000, SDS-PAGE	uncultured bacterium

Synonyms

Synonyms	Comment	Organism
Xyn-Lxy	-	uncultured bacterium

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
50	-	-	uncultured bacterium

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
125.9	-	birchwood xylan	pH 6.0, 50°C	uncultured bacterium

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
6	-	-	uncultured bacterium

pI Value

Organism	Comment	pI Value Maximum	pI Value
uncultured bacterium	calculated	-	5

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Release 2023.1 (January 2023)