Crystallization (Comment) | Organism |
---|---|
structure of a truncated derivative of comprising exclusively the enzyme catalytic module. The enzyme displays a (beta/alpha)8 TIM-barrel core with a side-associated beta-sheet domain. In mutant E225A, solved in the presence of xylotetraose, xylotetraose-cleavage oligosaccharides partially occupy subsites -3 to +2. The sugar ring at the +1 subsite is held in place by hydrophobic stacking interactions between Tyr139 and Tyr200 and hydrogen bonds to the OH group of Tyr227. The xylopyranosyl ring at the -1 subsite appears in the alpha-anomeric configuration | Acetivibrio thermocellus |
Protein Variants | Comment | Organism |
---|---|---|
E225A | mutation in catalytic residue, crystallization data | Acetivibrio thermocellus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cellulosome | - |
Acetivibrio thermocellus | 43263 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
45000 | - |
- |
Acetivibrio thermocellus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Acetivibrio thermocellus | A3DJS9 | - |
- |
Acetivibrio thermocellus DSM 1237 | A3DJS9 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | acts predominantly on polysaccharides with xylan as the main chain and substituted with varying amounts of glucuronic acids, such as in beechwood xylan, birchwood xylan and 4-O-methylglucuronoxylan | Acetivibrio thermocellus | ? | - |
? | |
additional information | acts predominantly on polysaccharides with xylan as the main chain and substituted with varying amounts of glucuronic acids, such as in beechwood xylan, birchwood xylan and 4-O-methylglucuronoxylan | Acetivibrio thermocellus DSM 1237 | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 45000, calcuated | Acetivibrio thermocellus |
Synonyms | Comment | Organism |
---|---|---|
Cthe_3012 | - |
Acetivibrio thermocellus |
Xyn30A | - |
Acetivibrio thermocellus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
75 | - |
- |
Acetivibrio thermocellus |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | 70 | more than 95% of maximum activity | Acetivibrio thermocellus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6 | - |
- |
Acetivibrio thermocellus |