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Literature summary for 3.2.1.8 extracted from
- An acid and highly thermostable xylanase from Phialophora sp. G5 (2011), Appl. Microbiol. Biotechnol., 89, 1851-1858.
Application
| Application | Comment | Organism |
|---|---|---|
| industry | XYN10G5 a good candidate for application in the animal feed industry | Phialophora sp. CGMCC 3328 |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| gene xyn10G5, DNA and amino acid sequence determination and analysis, expression in Pichia pastoris strain GS115 | Phialophora sp. CGMCC 3328 |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Ag+ | complete inhibition of XYN10G at 5 mM | Phialophora sp. CGMCC 3328 |  |
| Co2+ | strong inhibition of XYN10G at 1 mM | Phialophora sp. CGMCC 3328 | |
| Cu2+ | strong inhibition of XYN10G at 1 mM | Phialophora sp. CGMCC 3328 | |
| Hg2+ | complete inhibition of XYN10G at 5 mM | Phialophora sp. CGMCC 3328 | |
| additional information | after treatment with pepsin and trypsin at 37°C for 1 h, XYN10G retains 64.7% and 100% activity, respectively | Phialophora sp. CGMCC 3328 | |
| Ni2+ | strong inhibition of XYN10G at 1 mM | Phialophora sp. CGMCC 3328 | |
| SDS | complete inhibition of XYN10G at 5 mM | Phialophora sp. CGMCC 3328 | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| additional information | Na+, K+, Li+, Ca2+, Mg2+, Mn2+ and EDTA, have little or no effect on the enzyme activity | Phialophora sp. CGMCC 3328 |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 40000 | - |
x * 55000, recombinant enzyme, SDS-PAGE, x * 40000, about, sequence calculation | Phialophora sp. CGMCC 3328 |
| 55000 | - |
x * 55000, recombinant enzyme, SDS-PAGE, x * 40000, about, sequence calculation | Phialophora sp. CGMCC 3328 |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Phialophora sp. CGMCC 3328 | F2VRY7 | gene xyn10G5 | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant enzyme from Pichia pastoris strain GS115 by ultrafiltration, anion exchange chromatography, and gel filtration | Phialophora sp. CGMCC 3328 |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 207.2 | - |
purified recombinant XYN10G5, substrate oat spelt xylan, pH 4.0, 70°C | Phialophora sp. CGMCC 3328 |
| 350.6 | - |
purified recombinant XYN10G5, substrate soluble wheat arabinoxylan, pH 4.0, 70°C | Phialophora sp. CGMCC 3328 |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| birch wood xylan + H2O | - |
Phialophora sp. CGMCC 3328 | ? | - |
? | |
| additional information | composition of the hydrolysis products from oat spelt xylan, birchwood xylan, and soluble wheat arabinoxylan are 43.3% xylose and 56.7% xylobiose, 41.0% xylose and 59.0% xylobiose, and 42.4% xylose, and 57.6% xylobiose, respectively | Phialophora sp. CGMCC 3328 | ? | - |
? | |
| oat spelt xylan + H2O | - |
Phialophora sp. CGMCC 3328 | ? | - |
? | |
| soluble wheat arabinoxylan + H2O | best substrate of XYN10G5, Under simulated gastric conditions, XYN10G5 is stable and releases more reducing sugars from soluble wheat arabinoxylan | Phialophora sp. CGMCC 3328 | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 55000, recombinant enzyme, SDS-PAGE, x * 40000, about, sequence calculation | Phialophora sp. CGMCC 3328 |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| endo-beta-1,4-xylanase | - |
Phialophora sp. CGMCC 3328 |
| More | the enzyme belongs to the glycoside hydrolase domain family 10 | Phialophora sp. CGMCC 3328 |
| XYN10G5 | - |
Phialophora sp. CGMCC 3328 |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 70 | - |
- |
Phialophora sp. CGMCC 3328 |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 70 | - |
XYN10G, about 90% of activity remaining after 1 h, 37°C | Phialophora sp. CGMCC 3328 |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 4 | - |
- |
Phialophora sp. CGMCC 3328 |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 3 | 9 | XYN10G, 70% of maximal activity within this range | Phialophora sp. CGMCC 3328 |
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