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Literature summary for 3.2.1.8 extracted from

  • Ghaffar, A.; Khan, S.A.; Mukhtar, Z.; Rajoka, M.I.; Latif, F.
    Heterologous expression of a gene for thermostable xylanase from Chaetomium thermophilum in Pichia pastoris GS115 (2011), Mol. Biol. Rep., 38, 3227-3233.
    View publication on PubMed
Search for more literature for 3.2.1.8

Cloned(Commentary)

Cloned (Comment) Organism
gene xyn11A, DNA and amino acid sequence determination and analysis, subcloning in Escherichia coli, expression of the gene with intron, and the gene without intron under the control of AOX1 promoter in Pichia pastoris strain GS115, the enzyme is secreted to the culture medium Thermochaetoides thermophila

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular
-
 Thermochaetoides thermophila
-
-

Organism

Organism UniProt Comment Textmining
Thermochaetoides thermophila Q8J1V6 gene xyn11A
-
Thermochaetoides thermophila NIBGE 1 Q8J1V6 gene xyn11A
-

Source Tissue

Source Tissue Comment Organism Textmining
mycelium
-
 Thermochaetoides thermophila
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
 recombinant xylanase from gene xyn698 with intron gives 15.6 U/ml, that of gene xyn699 without intron results in 1.26 U/ml after 96 h growth Thermochaetoides thermophila

Synonyms

Synonyms Comment Organism
beta-1,4-xylanase
-
 Thermochaetoides thermophila
xylanase
-
 Thermochaetoides thermophila

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
70
-
-
 Thermochaetoides thermophila

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
30
-
 purified recombinant enzyme expressed from gene with intron, half-life is 1959 min Thermochaetoides thermophila
40
-
 purified recombinant enzyme expressed from gene with intron, half-life is 959 min Thermochaetoides thermophila
45
-
 purified recombinant enzyme expressed from gene with intron, 10 min, 100% activity remaining Thermochaetoides thermophila
50
-
 purified recombinant enzyme expressed from gene with intron, half-life is 176 min Thermochaetoides thermophila
60
-
 purified recombinant enzyme expressed from gene with intron, half-life is 72 min Thermochaetoides thermophila
70
-
 purified recombinant enzyme expressed from gene with intron, half-life is 39.4 min Thermochaetoides thermophila
80
-
 purified recombinant enzyme expressed from gene with intron, half-life is 18.7 min Thermochaetoides thermophila

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.5
-
-
 Thermochaetoides thermophila

pH Stability

pH Stability pH Stability Maximum Comment Organism
5.5 8.5 purified recombinant enzyme, quite stable Thermochaetoides thermophila