Cloned (Comment) | Organism |
---|---|
gene Kxyn, DNA and amino acid sequence determination and analysis, subcloning in Escherichia coli strain DH5alpha, expression in Escherichia coli strain BL21 (DE3) | Kocuria sp. Mn22 |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Co2+ | - |
Kocuria sp. Mn22 | |
Cu2+ | - |
Kocuria sp. Mn22 | |
Fe2+ | - |
Kocuria sp. Mn22 | |
Hg2+ | - |
Kocuria sp. Mn22 | |
Mn2+ | - |
Kocuria sp. Mn22 | |
additional information | Kxyn is not affected by 2-mercaptoethanol, DTT, or SDS | Kocuria sp. Mn22 | |
Pb2+ | - |
Kocuria sp. Mn22 | |
Zn2+ | - |
Kocuria sp. Mn22 |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | Km for beta-1,4-xylan from birchwood is 5.4 mg/ml | Kocuria sp. Mn22 |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
additional information | Kxyn is not affected by Ca2+, Mg2+, Na+, K+ | Kocuria sp. Mn22 |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
beta-1,4-xylan + H2O | Kocuria sp. Mn22 | - |
xylobiose + xylotriose + ? | predominant products | ? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Kocuria sp. Mn22 | B8XY24 | gene Kxyn | - |
Purification (Comment) | Organism |
---|---|
recombinant enzyme from Escherichia coli strain BL21 (DE3) | Kocuria sp. Mn22 |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
beta-1,4-xylan + H2O | - |
Kocuria sp. Mn22 | xylobiose + xylotriose + ? | predominant products | ? | |
beta-1,4-xylan + H2O | from birchwood | Kocuria sp. Mn22 | xylobiose + xylotriose + ? | predominant products | ? |
Synonyms | Comment | Organism |
---|---|---|
endo-beta-1,4-xylanase | - |
Kocuria sp. Mn22 |
More | the enzyme belongs to the glycosyl hydrolase family 10, GH10 | Kocuria sp. Mn22 |
xylanase | - |
Kocuria sp. Mn22 |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
55 | - |
- |
Kocuria sp. Mn22 |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | 70 | activity range | Kocuria sp. Mn22 |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
55 | - |
purified recombinant enzyme, 60 min, 70% activity remaining | Kocuria sp. Mn22 |
60 | - |
purified recombinant enzyme, 60 min, 30% activity remaining | Kocuria sp. Mn22 |
70 | - |
purified recombinant enzyme, 60 min, 10% activity remaining | Kocuria sp. Mn22 |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
185.1 | - |
beta-1,4-xylan | pH 8.5, 55°C, birchwood xylan | Kocuria sp. Mn22 |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.5 | - |
- |
Kocuria sp. Mn22 |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6 | 12 | activity range | Kocuria sp. Mn22 |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
7.6 | 12 | purified recombinant enzyme, over 80% remaining activity after overnight incubation | Kocuria sp. Mn22 |