Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.2.1.8 extracted from

  • Sajjad, M.; Khan, M.I.; Akbar, N.S.; Ahmad, S.; Ali, I.; Akhtar, M.W.
    Enhanced expression and activity yields of Clostridium thermocellum xylanases without non-catalytic domains (2010), J. Biotechnol., 145, 38-42.
    View publication on PubMed
Search for more literature for 3.2.1.8

Cloned(Commentary)

Cloned (Comment) Organism
gene xynC, subcloning in Escherichia coli strain DH5alpha, expression of wild-type and mutant enzymes in Escherichia coli Acetivibrio thermocellus
gene xynZ, subcloning in Escherichia coli strain DH5alpha, expression of wild-type and mutant enzymes in Escherichia coli Acetivibrio thermocellus

Protein Variants

Protein Variants Comment Organism
additional information generation of two constructs of XynC, one with its cellulose binding domain and the catalytic domain, pXynC-BC, and the other with only the catalytic domain, pXynC-C. The specific activities of XynC with and without the non-catalytic domains are similar Acetivibrio thermocellus
additional information generation of two constructs of XynZ: pXynZ-BDC, which includes the dockerin domain, and pXynZ-C, which does not. For XynZ, the specific activity of the enzyme without the non-catalytic domains is about 5fold greater than that of the intact enzyme. The overall increase in activity is 9fold higher for XynZ-C versus XynZ-BDC Acetivibrio thermocellus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information Km values for mutants XynC-BC and XynC-C determined on soluble xylan are 3.1 mg/ml and 3.6 mg/ml, respectively Acetivibrio thermocellus
additional information
-
 additional information Km values for mutants XynZ-BDC and XynZ-C, determined on soluble xylan, are 33.3 mg/ml and 15.4 mg/ml, respectively Acetivibrio thermocellus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
beta-1,4-xylan + H2O Acetivibrio thermocellus
-
 ?
-
 ?

Organism

Organism UniProt Comment Textmining
Acetivibrio thermocellus O32374 xynC; strain ATCC 27405D, gene xynC
-
Acetivibrio thermocellus P10478 xynZ; strain ATCC 27405D, gene xynZ
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
 activities of wild-type XynC, and its domain deletion mutants, overview Acetivibrio thermocellus
additional information
-
 activities of wild-type XynZ, and its domain deletion mutants, overview Acetivibrio thermocellus
3 8 purified mutant XynC-BC Acetivibrio thermocellus
39
-
 purified mutant XynC-C Acetivibrio thermocellus
79
-
 purified mutant XynZ-BDC Acetivibrio thermocellus
390
-
 purified mutant XynZ-C Acetivibrio thermocellus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
beta-1,4-xylan + H2O
-
 Acetivibrio thermocellus ?
-
 ?
beta-1,4-xylan + H2O from birchwood Acetivibrio thermocellus ?
-
 ?
beta-1,4-xylan + H2O insoluble xylan from birchwood, and soluble xylan Acetivibrio thermocellus ?
-
 ?

Synonyms

Synonyms Comment Organism
xylanase
-
 Acetivibrio thermocellus
XynC
-
 Acetivibrio thermocellus
XynZ
-
 Acetivibrio thermocellus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
 assay at Acetivibrio thermocellus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
70
-
 2 h, 87% activity of mutant Xyn-BDC is remaining, 42% of mutant Xyn-BDC Acetivibrio thermocellus
70
-
 2 h, mutant Xyn-C loses all activity, while mutant XynC-BC retains 70% activity Acetivibrio thermocellus