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Literature summary for 3.2.1.8 extracted from

  • Luo, H.; Li, J.; Yang, J.; Wang, H.; Yang, Y.; Huang, H.; Shi, P.; Yuan, T.; Fan, Y.; Yao, B.
    A thermophilic and acid stable family-10 xylanase from the acidophilic fungus Bispora sp. MEY-1 (2009), Extremophiles, 13, 849-857.
    View publication on PubMed
Search for more literature for 3.2.1.8

Activating Compound

Activating Compound Comment Organism Structure
2-mercaptoethanol activates 16% at 10 mM Bispora sp. MEY-1

Cloned(Commentary)

Cloned (Comment) Organism
gene xyl10C from genomic DNA, DNA and amino acid sequence determination and analysis, sequence comparisons, overexpression in Pichia pastoris Bispora sp. MEY-1

Inhibitors

Inhibitors Comment Organism Structure
Ca2+
-
 Bispora sp. MEY-1
Cu2+
-
 Bispora sp. MEY-1
Fe3+
-
 Bispora sp. MEY-1
Mg2+
-
 Bispora sp. MEY-1
additional information poor inhibition by Ni2+ and Co2+ Bispora sp. MEY-1
Pb2+
-
 Bispora sp. MEY-1
SDS complete inhibition at 5 mM Bispora sp. MEY-1
Zn2+
-
 Bispora sp. MEY-1

Metals/Ions

Metals/Ions Comment Organism Structure
Ag+ activates 66.5% at 1 mM Bispora sp. MEY-1
Cr3+ activates 19% at 10 mM Bispora sp. MEY-1
additional information no effect by EDTA, Li+, Na+, and K+ Bispora sp. MEY-1

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
55000
-
 x * 449000, Xyl10C, sequence calculation, x * 90000, about, glycosylated recombinant Xyl10C, SDS-PAGE, x * 55000, deglycosylated recombinant Xyl10C, SDS-PAGE Bispora sp. MEY-1
90000
-
 x * 449000, Xyl10C, sequence calculation, x * 90000, about, glycosylated recombinant Xyl10C, SDS-PAGE, x * 55000, deglycosylated recombinant Xyl10C, SDS-PAGE Bispora sp. MEY-1
449000
-
 x * 449000, Xyl10C, sequence calculation, x * 90000, about, glycosylated recombinant Xyl10C, SDS-PAGE, x * 55000, deglycosylated recombinant Xyl10C, SDS-PAGE Bispora sp. MEY-1

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
beta-1,4-xylan + H2O Bispora sp. MEY-1
-
 ?
-
 ?

Organism

Organism UniProt Comment Textmining
Bispora sp. MEY-1 D0QF43 CGMCC 2500, gene xyl10C
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein
-
 Bispora sp. MEY-1

Purification (Commentary)

Purification (Comment) Organism
recombinant Xyl10C 1.1fold from Pichia pastoris by anion exchange chromatography Bispora sp. MEY-1

Source Tissue

Source Tissue Comment Organism Textmining
mycelium grown on wheat bran Bispora sp. MEY-1
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
18830
-
 purified recombinant Xyl10C Bispora sp. MEY-1

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
beta-1,4-xylan + H2O
-
 Bispora sp. MEY-1 ?
-
 ?
beta-1,4-xylan + H2O from oat spelt Bispora sp. MEY-1 ?
-
 ?

Subunits

Subunits Comment Organism
? x * 449000, Xyl10C, sequence calculation, x * 90000, about, glycosylated recombinant Xyl10C, SDS-PAGE, x * 55000, deglycosylated recombinant Xyl10C, SDS-PAGE Bispora sp. MEY-1

Synonyms

Synonyms Comment Organism
endo-1,4-beta-xylanase
-
 Bispora sp. MEY-1
More the enzyme belongs to the glycosyl hydrolase family 10, GH10 Bispora sp. MEY-1
XYL10C
-
 Bispora sp. MEY-1

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
85
-
 recombinant Xyl10C Bispora sp. MEY-1

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
35 100 activity range, 80% of maximal activity at 75-90°C, 20% at 100°C, and 10% at 40°C, profile, overview Bispora sp. MEY-1

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
80
-
 purified recombinant Xyl10C, 60 min, completely stablem half-life is 45 h Bispora sp. MEY-1
85
-
 purified recombinant Xyl10C, half-life is 3 h Bispora sp. MEY-1
90
-
 purified recombinant Xyl10C, 10 min, 87% remaining activity Bispora sp. MEY-1

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
4.5 5 recombinant Xyl10C Bispora sp. MEY-1

pH Range

pH Minimum pH Maximum Comment Organism
2 7 first peak at pH 3.0 with 63% of maximal activity, and second peak with maximal activity at pH 4.5-5.0, profile, overview Bispora sp. MEY-1