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Literature summary for 3.2.1.8 extracted from

  • Jun, H.; Bing, Y.; Keying, Z.; Daiwen, C.
    Functional characterization of a recombinant thermostable xylanase from Pichia pastoris: A hybrid enzyme being suitable for xylooligosaccharides production (2009), Biochem. Eng. J., 48, 87-92.
No PubMed abstract available

Cloned(Commentary)

Cloned (Comment) Organism
gene xyn2, expressionin Pichia pastoris Trichoderma reesei

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
additional information kinetics Trichoderma reesei

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
beta-1,4-xylan + H2O Trichoderma reesei
-
?
-
?

Organism

Organism UniProt Comment Textmining
Trichoderma reesei
-
gene xyn2
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
beta-1,4-xylan + H2O
-
Trichoderma reesei ?
-
?
beta-1,4-xylan + H2O from oat spelt, birchwood, or beechwood Trichoderma reesei ?
-
?

Synonyms

Synonyms Comment Organism
Xyn2
-
Trichoderma reesei

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
65
-
recombinant enzyme Trichoderma reesei

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
30 90 recombinant enzyme, activity range, 45% of maximal activity at 80°C, profile, overview Trichoderma reesei

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
70
-
purified recombinant enzyme, loss of 60% activity after 30 min Trichoderma reesei

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
168.2
-
beta-1,4-xylan recombinant enzyme, pH 6.0, 50°C, birchwood xylan Trichoderma reesei
193.6
-
beta-1,4-xylan recombinant enzyme, pH 6.0, 50°C, oat spelt xylan Trichoderma reesei

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6
-
recombinant enzyme Trichoderma reesei

pH Range

pH Minimum pH Maximum Comment Organism
2 9 recombinant enzyme, profile, overview Trichoderma reesei