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Literature summary for 3.2.1.8 extracted from

  • Luo, H.; Yang, J.; Li, J.; Shi, P.; Huang, H.; Bai, Y.; Fan, Y.; Yao, B.
    Molecular cloning and characterization of the novel acidic xylanase XYLD from Bispora sp. MEY-1 that is homologous to family 30 glycosyl hydrolases (2010), Appl. Microbiol. Biotechnol., 86, 1829-1839.
    View publication on PubMed
Search for more literature for 3.2.1.8

Activating Compound

Activating Compound Comment Organism Structure
2-mercaptoethanol
-
 Bispora sp. MEY-1

Cloned(Commentary)

Cloned (Comment) Organism
gene xylD, DNA and amino acid sequence determination and analysis, phylogenetic tree, subcloning in Escherichia coli strain DH10B, expression in Pichia pastoris GS115 Bispora sp. MEY-1

Inhibitors

Inhibitors Comment Organism Structure
Cu2+
-
 Bispora sp. MEY-1
Fe3+
-
 Bispora sp. MEY-1
Hg2+
-
 Bispora sp. MEY-1
Li+
-
 Bispora sp. MEY-1
Mn2+
-
 Bispora sp. MEY-1
additional information XYLD shows resistant to pepsin and part resistant to trypsin Bispora sp. MEY-1
Pb2+
-
 Bispora sp. MEY-1

Metals/Ions

Metals/Ions Comment Organism Structure
Ni2+ activates Bispora sp. MEY-1

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
49800
-
 x * 49800, about, sequence calculation, mature enzyme protein Bispora sp. MEY-1

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
beta-1,4-xylan + H2O Bispora sp. MEY-1
-
 ?
-
 ?

Organism

Organism UniProt Comment Textmining
Bispora sp. MEY-1 D6MYS9 isolated from acidic wastewater of a uranium mine in Jiangxi, China, gene xylD
-

Source Tissue

Source Tissue Comment Organism Textmining
additional information cultured in wheat bran medium, optimal growth occurs at pH 2.5-3.0 Bispora sp. MEY-1
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1429
-
 recombinant enzyme, substrate oat spelt xylan Bispora sp. MEY-1
2020
-
 recombinant enzyme, substrate 4-O-methyl-D-glucuronoxylan Bispora sp. MEY-1
2144
-
 recombinant enzyme, substrate birchwood xylan Bispora sp. MEY-1
2463
-
 recombinant enzyme, substrate beechwood xylan Bispora sp. MEY-1

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-nitrophenyl beta-D-xylose + H2O
-
 Bispora sp. MEY-1 4-nitrophenol + D-xylose
-
 ?
4-O-methyl-D-glucuronoxylan + H2O
-
 Bispora sp. MEY-1 ?
-
 ?
beta-1,4-xylan + H2O
-
 Bispora sp. MEY-1 ?
-
 ?
beta-1,4-xylan + H2O beechwood xylan, birchwood xylan, 4-O-methyl-D-glucuronoxylan, and oat spelt xylan Bispora sp. MEY-1 ?
-
 ?

Subunits

Subunits Comment Organism
? x * 49800, about, sequence calculation, mature enzyme protein Bispora sp. MEY-1

Synonyms

Synonyms Comment Organism
More XylD shows high sequence similarity to both glycosyl hydrolase families 5 and 30 but is more homologous to members of GH 30 based on phylogenetic analysis Bispora sp. MEY-1
XYLD
-
 Bispora sp. MEY-1

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
60
-
-
 Bispora sp. MEY-1

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
20 80
-
 Bispora sp. MEY-1

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
60
-
 purified recombinant enzyme, 60 min, pH 3.0, 98% remaining activity Bispora sp. MEY-1
80
-
 purified recombinant enzyme, 5 min, pH 3.0, 25% remaining activity Bispora sp. MEY-1

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
3
-
-
 Bispora sp. MEY-1

pH Range

pH Minimum pH Maximum Comment Organism
1.5 4 60% of maximal activity at pH 1.5 and pH 4.0 Bispora sp. MEY-1

pH Stability

pH Stability pH Stability Maximum Comment Organism
1 6 purified recombinantg enzyme, 37°C, stable Bispora sp. MEY-1
8
-
 and above, 60 min, complete inactivation Bispora sp. MEY-1