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Literature summary for 3.2.1.8 extracted from
- Effect of glycosylation and additional domains on the thermostability of a family 10 xylanase produced by Thermopolyspora flexuosa (2010), Appl. Environ. Microbiol., 76, 356-360.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression of C-terminally His-tagged wild-type, full-length Xyn10A, and of the isolated catalytic domain. Binding of the C-terminal 6His tag to the surface of XYN10A xylanase probably prevents unfolding from the C-terminus | Thermopolyspora flexuosa |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| additional information | the C-terminal carbohydrate binding module has only a slight effect, whereas a polyhistidine tag increases the thermostability of XYN10A | Thermopolyspora flexuosa |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Thermopolyspora flexuosa | - |
- |
- |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| glycoprotein | the N-glycosylation site Asn26, carrying GlcNAc(Man) as a glycan core structure, is located in a well-exposed loop, amino acids 21 to 28, between a beta-strand, amino acids 15 to 20, and alpha-helix, amino acids 29 to 37. Glycosylation can increase the thermostability. Glycosylation in a well-exposed loop in XYN10A xylanase can increase local mobility or destabilize the enzyme by affecting the local conformation | Thermopolyspora flexuosa |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| More | modeled structure of full-length XYN10A xylanase, overview | Thermopolyspora flexuosa |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| More | the enzyme belongs to the glycosyl hydrolase family 10, GH10 | Thermopolyspora flexuosa |
| xylanase | - |
Thermopolyspora flexuosa |
| Xyn10A | - |
Thermopolyspora flexuosa |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 70 | - |
recombinant His-tagged enzyme | Thermopolyspora flexuosa |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | 85 | recombinant His-tagged enzyme, inactive at 90°C | Thermopolyspora flexuosa |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| additional information | - |
the C-terminal carbohydrate binding module has only a slight effect, whereas a polyhistidine tag increases the thermostability of XYN10A | Thermopolyspora flexuosa |
| 65 | - |
recombinant His-tagged enzyme, 30 min, completely stable up to | Thermopolyspora flexuosa |
| 80 | - |
recombinant His-tagged enzyme, 30 min, 50% activity remaining | Thermopolyspora flexuosa |
| 95 | - |
recombinant His-tagged enzyme, 30 min, 10% activity remaining | Thermopolyspora flexuosa |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | - |
assay at | Thermopolyspora flexuosa |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 4 | 8.5 | - |
Thermopolyspora flexuosa |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 4 | - |
recombinant His-tagged enzyme, 30 min, 65°C, wild-type enzyme: half-life is 18 min with substrate, 3.1 min without substrate, isolated catalytic domain: life is 15 min with substrate, 1.3 min without substrate | Thermopolyspora flexuosa |
| 5.5 | - |
recombinant His-tagged enzyme, 30 min, 80°C, wild-type enzyme: half-life is 37 min with substrate, 19 min without substrate, isolated catalytic domain: life is 17 min with substrate, 33 min without substrate | Thermopolyspora flexuosa |
| 7 | - |
recombinant His-tagged enzyme, 30 min, 80°C, wild-type enzyme: half-life is 37 min with substrate, 23 min without substrate, isolated catalytic domain: life is 17 min with substrate, 22 min without substrate | Thermopolyspora flexuosa |
| 8.5 | - |
recombinant His-tagged enzyme, 30 min, 80°C, wild-type enzyme: half-life is 33 min with substrate, 23 min without substrate, isolated catalytic domain: life is 14 min with substrate, 26 min without substrate | Thermopolyspora flexuosa |
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