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Literature summary for 3.2.1.8 extracted from
- Optimized Expression of a Thermostable Xylanase 11 A Gene from Chaetomium thermophilum NIBGE 1 in Escherichia coli (2009), Protein Pept. Lett., 16, 356-362.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| additional information | xylose induces, is the best carbon source. Lactose is the best inducer, sucrose induces, glucose produces low levels of xylanase | Thermochaetoides thermophila |
Application
| Application | Comment | Organism |
|---|---|---|
| industry | is thermostable in alkaline buffer favoring its suitability to bio-bleaching of kraft pulp. Xylanases that are more active in pH range of 6.0-10.0 can act as promising agents in paper and pulp industry. Productivity profiles of xylanase in Escherichia coli recombinant are more than 4fold of that produced from Trichoderma reesei RUTC-30, 5fold of that produced by the donor and significantly higher than the values reported on other Escherichia coli, and Saccharomyces cerevisiae recombinants | Thermochaetoides thermophila |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| specific and exact size fragment cloned into vector pTZ57R for transformation into Escherichia coli DH5alpha. Cloned xyn11A gene excised from pTZ57R with HindIII and EcoRI and ligated into pET-32a (+) and expressed in Escherichia coli BL21 under T7 promoter | Thermochaetoides thermophila |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| additional information | removal of fusion protein rather decreases activity | Thermochaetoides thermophila |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 29300 | - |
1 * 29300, sequence analysis, 1 * 30000, SDS-PAGE | Thermochaetoides thermophila |
| 30000 | - |
1 * 29300, sequence analysis, 1 * 30000, SDS-PAGE | Thermochaetoides thermophila |
| 43000 | - |
fused xyn11A protein, Western blot analysis | Thermochaetoides thermophila |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Thermochaetoides thermophila | Q6UN40 | - |
- |
| Thermochaetoides thermophila NIBGE 1 | Q6UN40 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| enterokinase-treated enzyme purified by a combination of ammonium sulfate precipitation, gel filtration and ion exchange chromatography, to homogeneity | Thermochaetoides thermophila |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 32.6 | - |
enterokinase-digested endoxylanase | Thermochaetoides thermophila |
| 38.1 | - |
untreated endoxylanase | Thermochaetoides thermophila |
| 125 | - |
purified enterokinase-digested endoxylanase | Thermochaetoides thermophila |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | recombinant Xyn11A fails to hydrolyze CMC, avicel or p-nitrophenyl beta-xylopyranoside | Thermochaetoides thermophila | ? | - |
? |  |
| additional information | recombinant Xyn11A fails to hydrolyze CMC, avicel or p-nitrophenyl beta-xylopyranoside | Thermochaetoides thermophila NIBGE 1 | ? | - |
? | |
| oat spelt xylan + H2O | - |
Thermochaetoides thermophila | D-xylose + xylobiose + ? | - |
? | |
| oat spelt xylan + H2O | - |
Thermochaetoides thermophila NIBGE 1 | D-xylose + xylobiose + ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 29300, sequence analysis, 1 * 30000, SDS-PAGE | Thermochaetoides thermophila |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| endoxylanase | - |
Thermochaetoides thermophila |
| xylanase | - |
Thermochaetoides thermophila |
| xylanase 11 A | - |
Thermochaetoides thermophila |
| Xyn11A | - |
Thermochaetoides thermophila |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
- |
Thermochaetoides thermophila |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | 50 | - |
Thermochaetoides thermophila |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 80 | - |
retains 35% enzyme activity. The enzyme shows half lives of 1100, 330, 86, 70, 19.3 and 11.4 min at 30, 40, 50, 60, 70 and 80°C, respectively | Thermochaetoides thermophila |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.8 | - |
- |
Thermochaetoides thermophila |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 6 | 10 | recombinant enzyme | Thermochaetoides thermophila |
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