Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.2.1.8 extracted from

  • Ninawe, S.; Kapoor, M.; Kuhad, R.C.
    Purification and characterization of extracellular xylanase from Streptomyces cyaneus SN32 (2008), Biores. Technol., 99, 1252-1258.
    View publication on PubMed

Application

Application Comment Organism
paper production use in the pulp and paper industry for bleaching purposes Streptomyces cyaneus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
20500
-
SDS-PAGE Streptomyces cyaneus

Organism

Organism UniProt Comment Textmining
Streptomyces cyaneus
-
-
-
Streptomyces cyaneus SN32
-
-
-

Purification (Commentary)

Purification (Comment) Organism
ammonium sulfate precipitation and DEAE-Sepharose column chromatography Streptomyces cyaneus

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
893.6
-
after 2.25fold purification, at 60°C, in 0.2 M glycine-NaOH buffer, pH 9.0 Streptomyces cyaneus

Storage Stability

Storage Stability Organism
4°C, 50 mM Tris-HCl buffer (pH 7.5), more than 20 weeks, no loss of activity Streptomyces cyaneus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
birch wood xylan + H2O
-
Streptomyces cyaneus D-xylose + ?
-
?
birch wood xylan + H2O
-
Streptomyces cyaneus SN32 D-xylose + ?
-
?

Synonyms

Synonyms Comment Organism
xylanase
-
Streptomyces cyaneus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
60 65
-
Streptomyces cyaneus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
50 65 the half-lives of xylanase at 50°C and 65°C are approximately 200 and 50 min, respectively Streptomyces cyaneus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6
-
-
Streptomyces cyaneus

pH Stability

pH Stability pH Stability Maximum Comment Organism
4 9.5 at 60°C for 1 h, retaining 80% of its activity Streptomyces cyaneus
5 8 retains more than 60% of its activity Streptomyces cyaneus

pI Value

Organism Comment pI Value Maximum pI Value
Streptomyces cyaneus isoelectric focusing
-
8.5