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Literature summary for 3.2.1.8 extracted from

  • Van Campenhout, S.; Pollet, A.; Bourgois, T.M.; Rombouts, S.; Beaugrand, J.; Gebruers, K.; De Backer, E.; Courtin, C.M.; Delcour, J.A.; Volckaert, G.
    Unprocessed barley aleurone endo-beta-1,4-xylanase X-I is an active enzyme (2007), Biochem. Biophys. Res. Commun., 356, 799-804.
    View publication on PubMed
Search for more literature for 3.2.1.8

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli Hordeum vulgare

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
61500
-
 unprocessed active enzyme, SDS-PAGE Hordeum vulgare

Organism

Organism UniProt Comment Textmining
Hordeum vulgare Q94G06
-
-

Purification (Commentary)

Purification (Comment) Organism
His Trap HP column chromatography and HiPrep 26/60 Sephacryl S-100 gel filtration Hordeum vulgare

Source Tissue

Source Tissue Comment Organism Textmining

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
birchwood xylan + H2O
-
 Hordeum vulgare ?
-
 ?
oat spelt xylan + H2O
-
 Hordeum vulgare ?
-
 ?
wheat arabinoxylan + H2O
-
 Hordeum vulgare ?
-
 ?
xylohexaose + H2O rapid cleavage Hordeum vulgare 2 xylotriose xylohexaose was cleaved to produce mainly xylotriose and to a lesser extent xylobiose and xylotetraose ?
xylopentaose + H2O rapid cleavage Hordeum vulgare xylobiose + xylotriose
-
 ?
xylotetraose + H2O low activity Hordeum vulgare 2 xylobiose
-
 ?
xylotriose + H2O low activity Hordeum vulgare xylobiose + xylose
-
 ?

Synonyms

Synonyms Comment Organism
endo-beta-1,4-xylanase
-
 Hordeum vulgare
X-I
-
 Hordeum vulgare

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
40
-
-
 Hordeum vulgare

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.016
-
 oat spelt xylan in 48.5 mM citric acid and 103 mM disodium hydrogen phosphate, pH 5, at 40°C Hordeum vulgare
2
-
 birchwood xylan in 48.5 mM citric acid and 103 mM disodium hydrogen phosphate, pH 5, at 40°C Hordeum vulgare
47.01
-
 oat spelt xylan in 48.5 mM citric acid and 103 mM disodium hydrogen phosphate, pH 5, at 40°C Hordeum vulgare
72.07
-
 birchwood xylan in 48.5 mM citric acid and 103 mM disodium hydrogen phosphate, pH 5, at 40°C Hordeum vulgare

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5
-
-
 Hordeum vulgare

pI Value

Organism Comment pI Value Maximum pI Value
Hordeum vulgare isoelectric focusing
-
 6.5