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Literature summary for 3.2.1.8 extracted from

  • Jaenis, J.; Pulkkinen, P.; Rouvinen, J.; Vainiotalo, P.
    Determination of steady-state kinetic parameters for a xylanase-catalyzed hydrolysis of neutral underivatized xylooligosaccharides by mass spectrometry (2007), Anal. Biochem., 365, 165-173.
    View publication on PubMed

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.073
-
xylohexaose in 10 mM ammonium acetate buffer at pH 5.0 and 20°C Trichoderma reesei
0.136
-
xylopentaose in 10 mM ammonium acetate buffer at pH 5.0 and 20°C Trichoderma reesei

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
21000
-
around 21000 Da, SDS-PAGE Trichoderma reesei

Organism

Organism UniProt Comment Textmining
Trichoderma reesei
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information xylobiose is not hydrolyzed Trichoderma reesei ?
-
?
xylohexaose + H2O most preferred substrate of TRX II Trichoderma reesei xylotriose + xylotriose xylohexaose is hydrolyzed mainly to two xylotrioses, but considerable amounts of xylotetraose and xylobiose are also detected while only traceable amounts of xylopentaose are detected ?
xylopentaose + H2O
-
Trichoderma reesei xylotriose + xylobiose xylopentaose is hydrolyzed mainly to xylotriose and xylobiose with only minor amounts of xylotetraose ?
xylotetraose + H2O
-
Trichoderma reesei xylobiose + xylobiose xylotetraose is hydrolyzed mainly to two xylobioses, with only traceable amounts of xylotriose ?
xylotriose + H2O
-
Trichoderma reesei xylobiose + xylose
-
?

Synonyms

Synonyms Comment Organism
endo-1,4-beta-xylanase II
-
Trichoderma reesei
TRX II
-
Trichoderma reesei
xylanase
-
Trichoderma reesei

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.045
-
xylotriose in 10 mM ammonium acetate buffer at pH 5.0 and 20°C Trichoderma reesei
0.162
-
xylotetraose in 10 mM ammonium acetate buffer at pH 5.0 and 20°C Trichoderma reesei
50.3
-
xylopentaose in 10 mM ammonium acetate buffer at pH 5.0 and 20°C Trichoderma reesei
68
-
xylohexaose in 10 mM ammonium acetate buffer at pH 5.0 and 20°C Trichoderma reesei

pI Value

Organism Comment pI Value Maximum pI Value
Trichoderma reesei isoelectric focusing
-
9