Literature summary for 3.2.1.8 extracted from

Carmona, E.C.; Fialho, M.B.; Buchgnani, E.B.; Coelho, G.D.; Brocheto-Braga, M.R.; Jorge, J.A.
Production, purification and characterization of a minor form of xylanase from Aspergillus versicolor (2005), Proc. Biochem., 40, 359-364.

No PubMed abstract available

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Activating Compound

Activating Compound	Comment	Organism	Structure
dithiothreitol	stimulation	Aspergillus versicolor

Inhibitors

Inhibitors	Comment	Organism	Structure
Cu2+	2 mM, complete inhibition	Aspergillus versicolor
EDTA	2 mM, 62% residual activity	Aspergillus versicolor
Hg2+	2 mM, complete inhibition	Aspergillus versicolor
NH4Cl	2 mM, 87% residual activity	Aspergillus versicolor
sodium dodecylsulfate	2 mM, 22% residual activity	Aspergillus versicolor
Zn2+	2 mM, 56% residual activity	Aspergillus versicolor

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Mn2+	stimulation	Aspergillus versicolor

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
32000	-	gel filtration	Aspergillus versicolor

Organism

Organism	UniProt	Comment	Textmining
Aspergillus versicolor	-	xylanase II, minor enzyme form, growth in 1% wheat bran as carbon source	-

Posttranslational Modification

Posttranslational Modification	Comment	Organism
glycoprotein	14% carbohydrate content	Aspergillus versicolor

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
302	-	pH 6.0, 55°C	Aspergillus versicolor

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
xylan + H2O	birchwood xylan is preferred	Aspergillus versicolor	?	-	?

Subunits

Subunits	Comment	Organism
monomer	x * 32000, SDS-PAGE	Aspergillus versicolor

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
55	-	-	Aspergillus versicolor

Temperature Stability [°C]

Temperature Stability Minimum [°C]	Temperature Stability Maximum [°C]	Comment	Organism
60	-	half-life 1.7 min	Aspergillus versicolor

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
6	7	-	Aspergillus versicolor

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Release 2023.1 (January 2023)