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Literature summary for 3.2.1.8 extracted from
- Production, purification and properties of xylanase from a newly isolated Fusarium proliferatum (2002), Process Biochem., 37, 1279-1284.
No PubMed abstract available
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Cu2+ | - |
Fusarium proliferatum |  |
| DTT | - |
Fusarium proliferatum | |
| Mn2+ | - |
Fusarium proliferatum | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| extracellular | - |
Fusarium proliferatum | - |
- |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| additional information | no metal requirement for activity and stability | Fusarium proliferatum |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 22400 | - |
1 * 22400, SDS-PAGE | Fusarium proliferatum |
| 25000 | - |
gel filtration | Fusarium proliferatum |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Fusarium proliferatum | - |
- |
- |
| Fusarium proliferatum NRRL 26517 | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| 975fold from the culture supernatant, to homogeneity by ammonium sulfate precipitation and several chromatographic steps | Fusarium proliferatum |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 591 | - |
purified enzyme | Fusarium proliferatum |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| xylan + H2O | diverse xylan substrates, best substrate is wheat arabinoxylan, overview | Fusarium proliferatum | xylobiose + ? | no formation of xylose | ? | |
| xylan + H2O | diverse xylan substrates, best substrate is wheat arabinoxylan, overview | Fusarium proliferatum NRRL 26517 | xylobiose + ? | no formation of xylose | ? | |
| xylan + H2O | xylan substrate from oat spelt | Fusarium proliferatum | xylobiose + xylotetraose + ? | no formation of xylose | ? | |
| xylan + H2O | xylan substrate from oat spelt | Fusarium proliferatum NRRL 26517 | xylobiose + xylotetraose + ? | no formation of xylose | ? | |
| xylan + H2O | xylan substrate from birchwood | Fusarium proliferatum | xylobiose + xylotriose + ? | no formation of xylose | ? | |
| xylan + H2O | xylan substrate from birchwood | Fusarium proliferatum NRRL 26517 | xylobiose + xylotriose + ? | no formation of xylose | ? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 22400, SDS-PAGE | Fusarium proliferatum |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| xylanase | - |
Fusarium proliferatum |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 55 | - |
- |
Fusarium proliferatum |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 40 | 70 | 89% of maximal activity at 60°C and 14% at 70°C | Fusarium proliferatum |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 55 | - |
fully stable up to, 30 min, pH 5.0 | Fusarium proliferatum |
| 60 | - |
loss of 78% activity after 30 min at pH 5.0 | Fusarium proliferatum |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5 | 5.5 | - |
Fusarium proliferatum |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 5 | 7.5 | fully stable, 1 h at 40°C, and 30 min at 55°C | Fusarium proliferatum |
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