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Literature summary for 3.2.1.8 extracted from
- The recombinant xylanase B of Thermotoga maritima is highly xylan specific and produces exclusively xylobiose from xylans, a unique character for industrial applications (2004), J. Mol. Catal. B, 27, 207-213.
No PubMed abstract available
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| gene xynB, expression as His-tagged enzyme in Escherichia coli BL21 | Thermotoga maritima |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | - |
Thermotoga maritima |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Thermotoga maritima | - |
xylanase XynB, gene xynB | - |
| Thermotoga maritima MSB8 / DSM 3109 / ATCC 43589 | - |
xylanase XynB, gene xynB | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant His-tagged enzyme from Escherichia coli, 12.5fold to homogeneity by Ni2+-chelate affinity chromatography | Thermotoga maritima |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 12 | - |
substrate carboxymethylcellulose, purified recombinant enzyme | Thermotoga maritima |
| 30 | - |
substrate laminarin, purified recombinant enzyme | Thermotoga maritima |
| 64 | - |
substrate gellan gum, purified recombinant enzyme | Thermotoga maritima |
| 164 | - |
substrate lichenan, purified recombinant enzyme | Thermotoga maritima |
| 867 | - |
substrate birchwood xylan, purified recombinant enzyme | Thermotoga maritima |
| 1022 | - |
substrate oat spelt xylan, purified recombinant enzyme | Thermotoga maritima |
| 1099 | - |
substrate beechwood xylan, purified recombinant enzyme | Thermotoga maritima |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| carboxymethylcellulose + H2O | low activity, 1.4% activity compared to xylan | Thermotoga maritima | ? | - |
? |  |
| carboxymethylcellulose + H2O | low activity, 1.4% activity compared to xylan | Thermotoga maritima MSB8 / DSM 3109 / ATCC 43589 | ? | - |
? | |
| gellan gum + H2O | low activity, 7.3% activity compared to xylan | Thermotoga maritima | ? | - |
? | |
| gellan gum + H2O | low activity, 7.3% activity compared to xylan | Thermotoga maritima MSB8 / DSM 3109 / ATCC 43589 | ? | - |
? | |
| laminarin + H2O | low activity, 3.4% activity compared to xylan | Thermotoga maritima | ? | - |
? | |
| laminarin + H2O | low activity, 3.4% activity compared to xylan | Thermotoga maritima MSB8 / DSM 3109 / ATCC 43589 | ? | - |
? | |
| lichenan + H2O | low activity, 19% activity compared to xylan | Thermotoga maritima | ? | - |
? | |
| lichenan + H2O | low activity, 19% activity compared to xylan | Thermotoga maritima MSB8 / DSM 3109 / ATCC 43589 | ? | - |
? | |
| additional information | substrate specificity, no activity with Avicel, filter paper, starch, amylopectin, pullulan, galactoglucomannan, or galactomannan | Thermotoga maritima | ? | - |
? | |
| additional information | substrate specificity, no activity with Avicel, filter paper, starch, amylopectin, pullulan, galactoglucomannan, or galactomannan | Thermotoga maritima MSB8 / DSM 3109 / ATCC 43589 | ? | - |
? | |
| xylan + H2O | birchwood and soluble oat spelt xylan, endo-acting | Thermotoga maritima | xylobiose + xylose + ? | main degradation products | ? | |
| xylooligosaccharides + H2O | - |
Thermotoga maritima | xylobiose + xylose | predominantly xylobiose | ? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| xylanase B | - |
Thermotoga maritima |
| XynB | - |
Thermotoga maritima |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | - |
assay at | Thermotoga maritima |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 89.7 | - |
xylan | soluble from oat spelt, pH 6.1, 50°C | Thermotoga maritima | |
| 106.5 | - |
xylan | from birchwood, pH 6.1, 50°C | Thermotoga maritima | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6.1 | - |
assay at | Thermotoga maritima |
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