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Literature summary for 3.2.1.8 extracted from
- A novel family 8 xylanase, functional and physicochemical characterization (2002), J. Biol. Chem., 277, 35133-35139.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| DNA and amino acid sequence determination and analysis, overexpression in Escherichia coli BL21(DE3) | Pseudoalteromonas haloplanktis |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Cu2+ | 16% inhibition at 1 mM | Pseudoalteromonas haloplanktis |  |
| Hg2+ | 31% inhibition at 1 mM | Pseudoalteromonas haloplanktis | |
| Ni2+ | 6% inhibition at 1 mM | Pseudoalteromonas haloplanktis | |
| Zn2+ | 14% inhibition at 1 mM | Pseudoalteromonas haloplanktis | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | - |
Pseudoalteromonas haloplanktis |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| extracellular | - |
Pseudoalteromonas haloplanktis | - |
- |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 45982 | - |
x * 45982, mass spectrometry | Pseudoalteromonas haloplanktis |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | Pseudoalteromonas haloplanktis | unrooted evolutionary tree, overview | ? | - |
? | |
| xylan + H2O | Pseudoalteromonas haloplanktis | major constituent of plant hemicellulose forming the cell walls of hards woods, 15-30%, and softwoods, 7-10% | D-xylotriose + xylotetraose + ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Pseudoalteromonas haloplanktis | Q8RJN8 | gene xyl; psychrophilic enzyme, antarctic bacterium | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant enzyme from Escherichia coli, native enzyme 11fold by ultrafiltration and ion exchange chromatography | Pseudoalteromonas haloplanktis |
Reaction
| Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|
| (Xylbeta(1-4))n + H2O = (Xylbeta(1-4))n-m + (Xylbeta(1-4))m | the O-glycoside hydrolase catalyzes the random hydrolysis of internal beta-1,4-D-xylosidic linkages of xylan and xylooligosaccharides of maximal 4 residue chain length, inverse stereoselectivity | Pseudoalteromonas haloplanktis |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | unrooted evolutionary tree, overview | Pseudoalteromonas haloplanktis | ? | - |
? | |
| additional information | enzyme shows no cellulase, chitosanase, and lichenase activity | Pseudoalteromonas haloplanktis | ? | - |
? | |
| xylan + H2O | major constituent of plant hemicellulose forming the cell walls of hards woods, 15-30%, and softwoods, 7-10% | Pseudoalteromonas haloplanktis | D-xylotriose + xylotetraose + ? | - |
? | |
| xylan + H2O | from oat spelt and other sources, xylan substrate specificity overview, inverse stereoselectivity, enzyme possesses a large substrate binding cleft with at least 6 xylose-binding sites | Pseudoalteromonas haloplanktis | D-xylotriose + xylotetraose + ? | - |
? | |
| xylooligosaccharide + H2O | long-chain substrates, high activity | Pseudoalteromonas haloplanktis | D-xylotriose + xylotetraose | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 45982, mass spectrometry | Pseudoalteromonas haloplanktis |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| More | enzyme belongs to family 8 of glycosyl hydrolases | Pseudoalteromonas haloplanktis |
| xylanase | - |
Pseudoalteromonas haloplanktis |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| additional information | - |
psychrophilic enzyme with high activity at low temperatures and low thermal stability | Pseudoalteromonas haloplanktis |
| 25 | - |
cold-adapted enzyme | Pseudoalteromonas haloplanktis |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 5 | 35 | 60% of maximal activity at 5°C | Pseudoalteromonas haloplanktis |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| additional information | - |
psychrophilic enzyme with high activity at low temperatures and low thermal stability | Pseudoalteromonas haloplanktis |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 1247 | - |
xylan | pH 6.5, 25°C | Pseudoalteromonas haloplanktis | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5.3 | 8 | at 25°C | Pseudoalteromonas haloplanktis |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 6 | 9 | - |
Pseudoalteromonas haloplanktis |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Pseudoalteromonas haloplanktis | - |
- |
9.5 |
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