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Literature summary for 3.2.1.8 extracted from

  • Hurlbert, J.C.; Preston, J.F., 3rd
    Functional characterization of a novel xylanase from a corn strain of Erwinia chrysanthemi (2001), J. Bacteriol., 183, 2093-2100.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
gene xynA, expression in Escherichia coli BL21(DE3) Dickeya chrysanthemi

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
xylan + H2O Dickeya chrysanthemi enzyme might be responsible for rendering substrates for other depolymerizing enzymes instead of producing oligoxylosides for cellular metabolism ?
-
?

Organism

Organism UniProt Comment Textmining
Dickeya chrysanthemi
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gene xynA, strain D1, xylanase A
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Purification (Commentary)

Purification (Comment) Organism
recombinant enzyme from Escherichia coli Dickeya chrysanthemi

Reaction

Reaction Comment Organism Reaction ID
(Xylbeta(1-4))n + H2O = (Xylbeta(1-4))n-m + (Xylbeta(1-4))m substrate recognition and sites of hydrolysis on the xylan substrates Dickeya chrysanthemi

Source Tissue

Source Tissue Comment Organism Textmining
additional information organism is isolated from corn Dickeya chrysanthemi
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Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
substrate specificity, pH-dependent activity with different substrates, overview Dickeya chrysanthemi

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-deoxy-hexenuronosylxylan + H2O
-
Dickeya chrysanthemi ?
-
?
4-O-methyl-D-glucuronoxylan + H2O
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Dickeya chrysanthemi ?
-
?
additional information substrate specificity Dickeya chrysanthemi ?
-
?
xylan + H2O enzyme might be responsible for rendering substrates for other depolymerizing enzymes instead of producing oligoxylosides for cellular metabolism Dickeya chrysanthemi ?
-
?
xylan + H2O xylan from sweetgum, beech-, and birchwood, specific for xylan substrates wirth a preference for a low ratio of xylose to 4-O-methyl-D-glucuronic acid, requires 4-O-methyl-D-glucuronic acid residues for substrate recognition and/or cleavage of a beta-1,4-xylosidic bond, no clevagae of the internal xylan backbone between xylose residues Dickeya chrysanthemi oligoxylosides
-
?

Subunits

Subunits Comment Organism
More secondary structure analysis, enzyme contains 10-15% alpha-helices at pH 6.0 and 35°C, portion of alpha-helix is pH-dependent Dickeya chrysanthemi

Synonyms

Synonyms Comment Organism
beta-1,4-xylan hydrolase
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Dickeya chrysanthemi
xylanase
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Dickeya chrysanthemi

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
35
-
-
Dickeya chrysanthemi

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
25 60
-
Dickeya chrysanthemi

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
45
-
instable above, loss of secondary structure and activity Dickeya chrysanthemi

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6
-
-
Dickeya chrysanthemi

pH Range

pH Minimum pH Maximum Comment Organism
5 8
-
Dickeya chrysanthemi