Cloned (Comment) | Organism |
---|---|
gene xynA, expression in Escherichia coli BL21(DE3) | Dickeya chrysanthemi |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
xylan + H2O | Dickeya chrysanthemi | enzyme might be responsible for rendering substrates for other depolymerizing enzymes instead of producing oligoxylosides for cellular metabolism | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Dickeya chrysanthemi | - |
gene xynA, strain D1, xylanase A | - |
Purification (Comment) | Organism |
---|---|
recombinant enzyme from Escherichia coli | Dickeya chrysanthemi |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
(Xylbeta(1-4))n + H2O = (Xylbeta(1-4))n-m + (Xylbeta(1-4))m | substrate recognition and sites of hydrolysis on the xylan substrates | Dickeya chrysanthemi |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
additional information | organism is isolated from corn | Dickeya chrysanthemi | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
substrate specificity, pH-dependent activity with different substrates, overview | Dickeya chrysanthemi |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-deoxy-hexenuronosylxylan + H2O | - |
Dickeya chrysanthemi | ? | - |
? | |
4-O-methyl-D-glucuronoxylan + H2O | - |
Dickeya chrysanthemi | ? | - |
? | |
additional information | substrate specificity | Dickeya chrysanthemi | ? | - |
? | |
xylan + H2O | enzyme might be responsible for rendering substrates for other depolymerizing enzymes instead of producing oligoxylosides for cellular metabolism | Dickeya chrysanthemi | ? | - |
? | |
xylan + H2O | xylan from sweetgum, beech-, and birchwood, specific for xylan substrates wirth a preference for a low ratio of xylose to 4-O-methyl-D-glucuronic acid, requires 4-O-methyl-D-glucuronic acid residues for substrate recognition and/or cleavage of a beta-1,4-xylosidic bond, no clevagae of the internal xylan backbone between xylose residues | Dickeya chrysanthemi | oligoxylosides | - |
? |
Subunits | Comment | Organism |
---|---|---|
More | secondary structure analysis, enzyme contains 10-15% alpha-helices at pH 6.0 and 35°C, portion of alpha-helix is pH-dependent | Dickeya chrysanthemi |
Synonyms | Comment | Organism |
---|---|---|
beta-1,4-xylan hydrolase | - |
Dickeya chrysanthemi |
xylanase | - |
Dickeya chrysanthemi |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
35 | - |
- |
Dickeya chrysanthemi |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | 60 | - |
Dickeya chrysanthemi |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
45 | - |
instable above, loss of secondary structure and activity | Dickeya chrysanthemi |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6 | - |
- |
Dickeya chrysanthemi |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5 | 8 | - |
Dickeya chrysanthemi |