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Literature summary for 3.2.1.8 extracted from

  • Mansour, F.A.; Shereif, A.A.; Nour el-Dein, M.M.; Abou-Dobara, M.I.; Ball, A.S.
    Purification and characterization of xylanase from a thermophilic Streptomyces sp. K37 (2003), Acta Microbiol. Pol., 52, 159-172.
    View publication on PubMed

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
additional information
-
Streptomyces sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
24300
-
gel filtration Streptomyces sp.
26400
-
1 * 26400, SDS-PAGE Streptomyces sp.

Organism

Organism UniProt Comment Textmining
Streptomyces sp.
-
-
-
Streptomyces sp. K37
-
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein 73.97% carbohydrate content Streptomyces sp.

Purification (Commentary)

Purification (Comment) Organism
33.5fold to homogeneity by ultrafiltration, cation exchange chromatography, and gel filtration Streptomyces sp.

Reaction

Reaction Comment Organism Reaction ID
(Xylbeta(1-4))n + H2O = (Xylbeta(1-4))n-m + (Xylbeta(1-4))m mode of action and hydrolysis pattern Streptomyces sp.

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
Streptomyces sp.
128.8
-
purified enzyme Streptomyces sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
arabinogalactan + H2O
-
Streptomyces sp. ?
-
?
arabinogalactan + H2O
-
Streptomyces sp. K37 ?
-
?
additional information substrate specificity, no activity with cellulose, carboxymethylcellulose, 4-nitrophenyl beta-D-xylopyranoside, and 4-nitrophenyl beta-D-glucopyranoside Streptomyces sp. ?
-
?
additional information substrate specificity, no activity with cellulose, carboxymethylcellulose, 4-nitrophenyl beta-D-xylopyranoside, and 4-nitrophenyl beta-D-glucopyranoside Streptomyces sp. K37 ?
-
?
xylan + H2O from oat spelt or birchwood, no release of arabinose Streptomyces sp. ?
-
?
xylan + H2O from oat spelt or birchwood, no release of arabinose Streptomyces sp. K37 ?
-
?

Subunits

Subunits Comment Organism
monomer 1 * 26400, SDS-PAGE Streptomyces sp.

Synonyms

Synonyms Comment Organism
endoxylanase
-
Streptomyces sp.
xylanase
-
Streptomyces sp.

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
60
-
-
Streptomyces sp.

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
30 75 about 50% of maximal activity at 50°C and 75°C Streptomyces sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6
-
-
Streptomyces sp.

pH Range

pH Minimum pH Maximum Comment Organism
4 12 86% of maximal activity at pH 4.0, 97% at pH 8.0, 30% activity at pH 10.0-12.0 Streptomyces sp.