Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.2.1.8 extracted from

  • Ruiz-Arribas, A.; Fernandez-Abalos, J.M.; Sanchez, P.; Garda, A.L.; Santamaria, R.I.
    Overproduction, purification, and biochemical characterization of a xylanase (Xys1) from Streptomyces halstedii JM8 (1995), Appl. Environ. Microbiol., 61, 2414-2419.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.2.1.8

Cloned(Commentary)

Cloned (Comment) Organism
expression in Streptomyces lividans, Streptomyces albus and Streptomyces parvulus Streptomyces halstedii

Organism

Organism UniProt Comment Textmining
Streptomyces halstedii
-
-
-
Streptomyces halstedii JM8
-
-
-

Purification (Commentary)

Purification (Comment) Organism
two enzyme form: Xys1S and Xys1L Streptomyces halstedii

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1,4-beta-D-xylan + H2O larchwood xylan Streptomyces halstedii additional information xylobiose is the main product ?
1,4-beta-D-xylan + H2O oat spelt xylan Streptomyces halstedii additional information xylobiose is the main product ?
1,4-beta-D-xylan + H2O larchwood xylan Streptomyces halstedii JM8 additional information xylobiose is the main product ?
1,4-beta-D-xylan + H2O oat spelt xylan Streptomyces halstedii JM8 additional information xylobiose is the main product ?
lichenan + H2O no activity Streptomyces halstedii ?
-
 ?
lichenan + H2O no activity Streptomyces halstedii JM8 ?
-
 ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
60
-
-
 Streptomyces halstedii

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
30 80 30°C: about 30% of maximal activity of enzyme form Xys1L, about 50% of maximal activity of enzyme form Xys1S, 80°C: about 15% of maximal activity of enzyme form Xys1L, about 35% of maximal activity of enzyme form Xys1S Streptomyces halstedii

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
4 50 1 h, pH 6.3, stable Streptomyces halstedii

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.3
-
-
 Streptomyces halstedii

pH Stability

pH Stability pH Stability Maximum Comment Organism
3
-
 32°C, 1 h, complete inactivation Streptomyces halstedii
4 10 32°C, 1 h, at least 80% of the activity maintained Streptomyces halstedii