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Literature summary for 3.2.1.8 extracted from
- Cloning, expression in Streptomyces lividans and biochemical characterization of a thermostable endo-beta-1,4-xylanase of Thermomonospora alba ULJB1 with cellulose-binding ability (1997), Appl. Microbiol. Biotechnol., 48, 208-217.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| overexpression in Streptomyces lividans | Thermobifida alba |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | 10 mM, significant inhibition | Thermobifida alba |  |
| Cu2+ | - |
Thermobifida alba | |
| Hg2+ | 10 mM, significant inhibition | Thermobifida alba | |
| Zn2+ | 10 mM, significant inhibition | Thermobifida alba | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| extracellular | - |
Thermobifida alba | - |
- |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Thermobifida alba | - |
ULJB1 | - |
| Thermobifida alba ULJB1 | - |
ULJB1 | - |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| proteolytic modification | the protein contains a 38-amino-acid leader peptide with 6 Arg residues in its amino-terminal end, a catalytic domain and a cellulose-binding domain connected by a linker region rich in Pro and Gly. A 38000 Da enzyme form and a 48000 Da enzyme form are detected by SDS-PAGE. The 38000 Da enzyme form does not contain the cellulose-binding domain | Thermobifida alba |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Thermobifida alba |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 1,4-beta-D-xylan + H2O | - |
Thermobifida alba | additional information | xylobiose and traces of xylose | ? | |
| 1,4-beta-D-xylan + H2O | - |
Thermobifida alba ULJB1 | additional information | xylobiose and traces of xylose | ? | |
| lichenan + H2O | no activity | Thermobifida alba | ? | - |
? | |
| lichenan + H2O | no activity | Thermobifida alba ULJB1 | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| More | a 38000 Da enzyme form and a 48000 Da enzyme form are detected by SDS-PAGE. The 38000 Da enzyme form does not contain the cellulose-binding domain | Thermobifida alba |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 80 | - |
- |
Thermobifida alba |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | 100 | 50°C: about 40% of maximal activity, 100°C: about 65% of maximal activity | Thermobifida alba |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 20 | 70 | 1 h, stable | Thermobifida alba |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6.5 | - |
- |
Thermobifida alba |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 4.5 | 10 | pH 4.5: about 55% of maximal activity, pH 10.0: about 60% of maximal activity | Thermobifida alba |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 4 | 10 | 1 h, stable | Thermobifida alba |
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