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Literature summary for 3.2.1.8 extracted from

  • Nakajima, T.; Tsukamoto, K.I.; Watanabe, T.; Kainuma, K.; Matsuda, K.
    Purification and some properties of an endo-1,4-beta-D-xylanase from Streptomyces sp. (1984), J. Ferment. Technol., 62, 269-276.
No PubMed abstract available
Search for more literature for 3.2.1.8

Inhibitors

Inhibitors Comment Organism Structure
Hg2+
-
 Streptomyces sp.
iodoacetate
-
 Streptomyces sp.
Mn2+
-
 Streptomyces sp.
SDS
-
 Streptomyces sp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information
-
 Streptomyces sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
42000
-
 gel filtration Streptomyces sp.
44000
-
 1 * 44000, SDS-PAGE Streptomyces sp.

Organic Solvent Stability

Organic Solvent Comment Organism
Ethanol 10%, 50% loss of activity Streptomyces sp.
Methanol 25%, 92% loss of activity Streptomyces sp.

Organism

Organism UniProt Comment Textmining
Streptomyces sp.
-
-
-
Streptomyces sp. KT-23
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Streptomyces sp.

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
 Streptomyces sp.

Storage Stability

Storage Stability Organism
4°C, 0.1 M acetate buffer, pH 5.5, or phosphate buffer pH 7.0, stable for at least 6 months Streptomyces sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1,4-beta-D-xylan + H2O
-
 Streptomyces sp. additional information xylobiose and a small amount of xylose ?
1,4-beta-D-xylan + H2O
-
 Streptomyces sp. KT-23 additional information xylobiose and a small amount of xylose ?
arabinoxylan + H2O
-
 Streptomyces sp. additional information
-
 ?
arabinoxylan + H2O
-
 Streptomyces sp. KT-23 additional information
-
 ?
xylohexaose + H2O
-
 Streptomyces sp. additional information
-
 ?
xylooctaose + H2O
-
 Streptomyces sp. xylobiose + xylotriose + xylotetraose
-
 ?
xylopentaose + H2O
-
 Streptomyces sp. additional information
-
 ?
xylopentaose + H2O
-
 Streptomyces sp. KT-23 additional information
-
 ?
xylotetraose + H2O
-
 Streptomyces sp. additional information
-
 ?
xylotetraose + H2O
-
 Streptomyces sp. KT-23 additional information
-
 ?
xylotriose + H2O
-
 Streptomyces sp. xylobiose + xylose
-
 ?
xylotriose + H2O
-
 Streptomyces sp. KT-23 xylobiose + xylose
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 44000, SDS-PAGE Streptomyces sp.

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
55
-
-
 Streptomyces sp.

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
55
-
 10 min, stable up to Streptomyces sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.5 6
-
 Streptomyces sp.