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Literature summary for 3.2.1.8 extracted from

  • Okazaki, W.; Akiba, T.; Horikoshi, K.; Akahoshi, R.
    Purification and characterization of xylanases from alkalophilic thermophilic Bacillus spp. (1985), Agric. Biol. Chem., 49, 2033-2039.
No PubMed abstract available
Search for more literature for 3.2.1.8

Inhibitors

Inhibitors Comment Organism Structure
Cu2+
-
 Bacillus sp. (in: Bacteria)
Hg2+ 5 mM, enzyme component I and II of strain W1 and W2, completely inhibited Bacillus sp. (in: Bacteria)

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information
-
 Bacillus sp. (in: Bacteria)

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
48500
-
 enzyme component II from strain W1, gel filtration Bacillus sp. (in: Bacteria)
51000
-
 enzyme component II from strain W2, gel filtration Bacillus sp. (in: Bacteria)

Organism

Organism UniProt Comment Textmining
Bacillus sp. (in: Bacteria)
-
 strains W1 (JCM 2888) and W2 (JCM 2889)
-

Purification (Commentary)

Purification (Comment) Organism
-
 Bacillus sp. (in: Bacteria)

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
 Bacillus sp. (in: Bacteria)

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1,4-beta-D-xylan + H2O
-
 Bacillus sp. (in: Bacteria) additional information hydrolysis products formed by enzyme component I of strain W1 and W2 are xylobiose + xylotriose + xylotetraose + xylopentaose, xylose is not formed. Hydrolysis products formed by enzyme component I of strain W1 and W2 are xylose + xylobiose + xylotriose + xylopentaose, xylotetraose is not formed. Xylobiose is the predominant product ?
additional information transferase activity Bacillus sp. (in: Bacteria) ?
-
 ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
65
-
 enzyme component I from strain W1 and strain W2 Bacillus sp. (in: Bacteria)
70
-
-
 Bacillus sp. (in: Bacteria)

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
40
-
 pH 6.0, 10 min, enzyme component I from strain W1, stable up to Bacillus sp. (in: Bacteria)
50
-
 pH 6.0, 10 min, enzyme component I from strain W2, stable up to Bacillus sp. (in: Bacteria)
60
-
 pH 6.0, 10 min, enzyme component II from strain W1 and W2, stable up to Bacillus sp. (in: Bacteria)

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6
-
-
 Bacillus sp. (in: Bacteria)
7 9
-
 Bacillus sp. (in: Bacteria)

pH Stability

pH Stability pH Stability Maximum Comment Organism
4.5 10 1 h, 45°C, stable between pH 4.5 and 10.0, enzyme component I and II from strain W1 and W2, stable Bacillus sp. (in: Bacteria)