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Literature summary for 3.2.1.8 extracted from

  • Matsuo, M.; Yasui, T.
    Xylanase of Malbranchea pulchella var. sulfurea (1988), Methods Enzymol., 160, 671-674.
No PubMed abstract available

Inhibitors

Inhibitors Comment Organism Structure
Cu2+ 1 mM Malbranchea pulchella
Hg2+ 0.1 mM Malbranchea pulchella

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
21000
-
x * 21000, xylanase A, SDS-PAGE Malbranchea pulchella
30000
-
x * 30000, xylanase B, SDS-PAGE Malbranchea pulchella

Organism

Organism UniProt Comment Textmining
Malbranchea pulchella
-
var. sulfurea
-

Purification (Commentary)

Purification (Comment) Organism
-
Malbranchea pulchella

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
Malbranchea pulchella
1475
-
xylanase A Malbranchea pulchella
1582
-
xylanase B Malbranchea pulchella

Storage Stability

Storage Stability Organism
4°C, 30 days, stable Malbranchea pulchella

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1,4-beta-D-xylan + H2O
-
Malbranchea pulchella additional information
-
?
arabinoxylan + H2O
-
Malbranchea pulchella additional information xylobiose + arabinotetraose + smaller amounts of xylose, arabinoxylopentaose, xylotriose and traces of branched arabino-xylose oligosaccharides with a degree of polymerization from 4-9. Xylanase B produces less xylose but more xylotriose and xylotetraose than xylanase A ?
additional information xylanase B shows high glycosyltransferase activity Malbranchea pulchella ?
-
?
xylobiose + H2O no hydrolysis Malbranchea pulchella ?
-
?
xyloheptaose + H2O
-
Malbranchea pulchella xylobiose + xylotriose + xylotetraose
-
?
xylooctaose + H2O
-
Malbranchea pulchella xylobiose + xylotriose + xylotetraose
-
?
xylopentaose + H2O
-
Malbranchea pulchella additional information xylose + xylobiose + xylotriose ?
xylotetraose + H2O
-
Malbranchea pulchella additional information
-
?
xylotriose + H2O weak activity Malbranchea pulchella xylobiose + xylose
-
?

Subunits

Subunits Comment Organism
? x * 30000, xylanase B, SDS-PAGE Malbranchea pulchella
? x * 21000, xylanase A, SDS-PAGE Malbranchea pulchella

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
45
-
xylanase A and B Malbranchea pulchella
70
-
-
Malbranchea pulchella

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
40
-
1 h, xylanase A is stable, 25% loss of activity of xylanase B Malbranchea pulchella
50
-
pH 6.7, 30 min, stable Malbranchea pulchella

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
3.5
-
xylanase A Malbranchea pulchella
6 6.5
-
Malbranchea pulchella
6.5
-
xylanase B Malbranchea pulchella

pH Stability

pH Stability pH Stability Maximum Comment Organism
2.5 6 xylanase A, stable Malbranchea pulchella
3.5 8 xylanase B, stable Malbranchea pulchella
6.7
-
50°C, 30 min, stable Malbranchea pulchella