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Literature summary for 3.2.1.8 extracted from

  • Lappalainen, A.
    Purification and characterization of xylanolytic enzymes from Trichoderma reesei (1986), Biotechnol. Appl. Biochem., 8, 437-448.
No PubMed abstract available
Search for more literature for 3.2.1.8

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
23000
-
 x * 23000, xylanase II, SDS-PAGE Trichoderma reesei
32000
-
 x * 32000, xylanase I, SDS-PAGE Trichoderma reesei

Organism

Organism UniProt Comment Textmining
Trichoderma reesei
-
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein xylanase I contains 14% carbohydrate, xylanase II contains 8% carbohydrate Trichoderma reesei

Purification (Commentary)

Purification (Comment) Organism
xylanase I and II Trichoderma reesei

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
 Trichoderma reesei

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1,4-beta-D-xylan + H2O insoluble birch xylan Trichoderma reesei additional information xylobiose and higher xylo-oligomers as main hydrolysis products ?
1,4-beta-D-xylan + H2O oat hulls xylan Trichoderma reesei additional information xylobiose and higher xylo-oligomers as main hydrolysis products ?
beta-glucan + H2O
-
 Trichoderma reesei ?
-
 ?
carboxymethylcellulose + H2O slight activity with xylanase I, no activity with xylanase II Trichoderma reesei ?
-
 ?
glucomannan + H2O slight activity with xylanase I, no activity with xylanase II Trichoderma reesei ?
-
 ?
laminarin + H2O xylanase I Trichoderma reesei ?
-
 ?
O-acetyl-4-O-glucuronoxylan + H2O
-
 Trichoderma reesei ?
-
 ?
pachyman + H2O slight activity with xylanase I, no activity with xylanase II Trichoderma reesei ?
-
 ?
pustulan + H2O slight activity with xylanase I, no activity with xylanase II Trichoderma reesei ?
-
 ?
starch + H2O soluble starch Trichoderma reesei ?
-
 ?

Subunits

Subunits Comment Organism
? x * 32000, xylanase I, SDS-PAGE Trichoderma reesei
? x * 23000, xylanase II, SDS-PAGE Trichoderma reesei

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
40
-
 20 h, 10% loss of activity of xylanase I, xylanase II is stable Trichoderma reesei
50
-
 20 h, 27% loss of activity of xylanase I and II Trichoderma reesei
60
-
 20 h, 84% loss of activity of xylanase I, 76% loss of activity of xylanase II Trichoderma reesei
70
-
 20 h, complete inactivation Trichoderma reesei

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
4 5 xylanase I and II Trichoderma reesei