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Literature summary for 3.2.1.78 extracted from

  • Seesom, W.; Thongket, P.; Yamamoto, T.; Takenaka, S.; Sakamoto, T.; Sukhumsirichart, W.
    Purification, characterization, and overexpression of an endo-1,4-beta-mannanase from thermotolerant Bacillus sp. SWU60 (2017), World J. Microbiol. Biotechnol., 33, 53 .
    View publication on PubMed
Search for more literature for 3.2.1.78

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21 (DE3) cells Bacillus sp. SWU60

Inhibitors

Inhibitors Comment Organism Structure
Ag+ 96% residual activity at 1 mM Bacillus sp. SWU60
EDTA 78% residual activity at 1 mM Bacillus sp. SWU60
Hg2+ 4% residual activity at 1 mM Bacillus sp. SWU60
Mn2+ 67% residual activity at 1 mM Bacillus sp. SWU60
Na+ 87% residual activity at 1 mM Bacillus sp. SWU60

Metals/Ions

Metals/Ions Comment Organism Structure
Ba2+ 106% activity at 1 mM Bacillus sp. SWU60
Cd2+ 104% activity at 1 mM Bacillus sp. SWU60
Cu2+ 106% activity at 1 mM Bacillus sp. SWU60
additional information not influenced by Co2+ and Ni2+ Bacillus sp. SWU60

Organism

Organism UniProt Comment Textmining
Bacillus sp. SWU60 A0A0K2S4R5
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein
-
 Bacillus sp. SWU60

Purification (Commentary)

Purification (Comment) Organism
butyl-Toyopearl 650 M column chromatography, DEAE-Toyopearl 650 M column chromatography, Mono Q column chromatography, Mono S column chromatography, Superdex 75 gel filtration, and HisTrap column chromatography Bacillus sp. SWU60

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.3
-
 crude enzyme, at pH 6.0 and 60°C Bacillus sp. SWU60
14
-
 after 46.7fold purification, at pH 6.0 and 60°C Bacillus sp. SWU60

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
beta-1,4-mannan + H2O 87% activity compared to konjac glucomannan Bacillus sp. SWU60 mannose + mannobiose + mannotriose
-
 ?
carob galactomannan + H2O 56% activity compared to konjac glucomannan Bacillus sp. SWU60 ?
-
 ?
konjac glucomannan + H2O 100% activity Bacillus sp. SWU60 ?
-
 ?
locust bean gum + H2O
-
 Bacillus sp. SWU60 ?
-
 ?
additional information the enzyme does not show activity toward guar galactomannan, 4-nitrophenyl beta-D-mannopyranoside, mannobiose, birchwood xylan, or carboxymethyl cellulose Bacillus sp. SWU60 ?
-
 ?

Subunits

Subunits Comment Organism
? x * 38000, native purified enzyme, SDS-PAGE Bacillus sp. SWU60
? x * 54000, recombinant protein, SDS-PAGE Bacillus sp. SWU60

Synonyms

Synonyms Comment Organism
endo-1,4-beta-mannanase
-
 Bacillus sp. SWU60
ManS2
-
 Bacillus sp. SWU60

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
60
-
-
 Bacillus sp. SWU60

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
30 80 more than 60% activity between 30 and 80°C Bacillus sp. SWU60

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
30 60 the enzyme is stable up to 60°C for 1 h. At 70°C, the activity drops to less than 40% after 1h Bacillus sp. SWU60

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6
-
-
 Bacillus sp. SWU60

pH Range

pH Minimum pH Maximum Comment Organism
5 8 more than 60% activity between pH 5.0 and 8.0 Bacillus sp. SWU60

pH Stability

pH Stability pH Stability Maximum Comment Organism
5 9 the enzyme is stable at pH 5.0-9.0 at 4 °C for 16 h Bacillus sp. SWU60