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Literature summary for 3.2.1.78 extracted from

  • Eom, G.; Oh, J.; Park, J.; Jegal, J.; Song, J.
    Secretory production of enzymatically active endo-beta-1,4-mannanase from Bacillus subtilis by ABC exporter in Escherichia coli (2016), Process Biochem., 51, 999-1005 .
No PubMed abstract available

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli XL10-Gold cells Bacillus subtilis

General Stability

General Stability Organism
the fused signal sequence of metalloprotease PrtA does not affect the enzymatic properties of the enzyme Bacillus subtilis

Organism

Organism UniProt Comment Textmining
Bacillus subtilis
-
-
-
Bacillus subtilis WL7
-
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein
-
Bacillus subtilis

Purification (Commentary)

Purification (Comment) Organism
ammonium sulfate precipitation, DEAE-Sepharose column chromatography and Q-Sepharose column chromatography Bacillus subtilis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
locust bean gum + H2O
-
Bacillus subtilis ?
-
?
locust bean gum + H2O
-
Bacillus subtilis WL7 ?
-
?

Synonyms

Synonyms Comment Organism
endo-beta-1,4-mannanase
-
Bacillus subtilis

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
55
-
-
Bacillus subtilis

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
45 60 about 60% activity at 45°C, about 80% activity at 50°C, 100% activity at 55°C, about 80% activity at 60°C, about 20% activity at 65°C Bacillus subtilis

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
35 45 the relatively high thermostability is retained after 1 h in the temperature range of 35-45°C and the stability rapidly decreases above 60°C Bacillus subtilis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6
-
-
Bacillus subtilis

pH Range

pH Minimum pH Maximum Comment Organism
5 8 about 30% activity at pH 4.0, about 90% activity at pH 5.0, 100% activity at pH 6.0, about 90% activity at pH 7.0, about 70% activity at pH 8.0, about 20% activity at pH 9.0 Bacillus subtilis

pH Stability

pH Stability pH Stability Maximum Comment Organism
6 9 the enzyme is highly stable with 100% activity remaining after 1 h within a pH range of 6.0-9.0 and about 80-90% activity remaining after 1 h within a pH range of 4.0-5.0. No residual activity is detected at pH 3.0 Bacillus subtilis