Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli XL10-Gold cells | Bacillus subtilis |
General Stability | Organism |
---|---|
the fused signal sequence of metalloprotease PrtA does not affect the enzymatic properties of the enzyme | Bacillus subtilis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus subtilis | - |
- |
- |
Bacillus subtilis WL7 | - |
- |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
glycoprotein | - |
Bacillus subtilis |
Purification (Comment) | Organism |
---|---|
ammonium sulfate precipitation, DEAE-Sepharose column chromatography and Q-Sepharose column chromatography | Bacillus subtilis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
locust bean gum + H2O | - |
Bacillus subtilis | ? | - |
? | |
locust bean gum + H2O | - |
Bacillus subtilis WL7 | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
endo-beta-1,4-mannanase | - |
Bacillus subtilis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
55 | - |
- |
Bacillus subtilis |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
45 | 60 | about 60% activity at 45°C, about 80% activity at 50°C, 100% activity at 55°C, about 80% activity at 60°C, about 20% activity at 65°C | Bacillus subtilis |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
35 | 45 | the relatively high thermostability is retained after 1 h in the temperature range of 35-45°C and the stability rapidly decreases above 60°C | Bacillus subtilis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6 | - |
- |
Bacillus subtilis |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5 | 8 | about 30% activity at pH 4.0, about 90% activity at pH 5.0, 100% activity at pH 6.0, about 90% activity at pH 7.0, about 70% activity at pH 8.0, about 20% activity at pH 9.0 | Bacillus subtilis |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
6 | 9 | the enzyme is highly stable with 100% activity remaining after 1 h within a pH range of 6.0-9.0 and about 80-90% activity remaining after 1 h within a pH range of 4.0-5.0. No residual activity is detected at pH 3.0 | Bacillus subtilis |