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Literature summary for 3.2.1.78 extracted from

  • Sakai, K.; Kimoto, S.; Shinzawa, Y.; Minezawa, M.; Suzuki, K.; Jindou, S.; Kato, M.; Shimizu, M.
    Characterization of pH-tolerant and thermostable GH 134 beta-1,4-mannanase SsGH134 possessing carbohydrate binding module 10 from Streptomyces sp. NRRL B-24484 (2018), J. Biosci. Bioeng., 125, 287-294 .
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21 CodonPlus(DE3) cells Streptomyces sp. NRRL B-24484

General Stability

General Stability Organism
although deleting carbohydrate binding module 10 increases the catalytic efficiency, its disruption decreases the pH, solvent and detergent stability of the enzyme Streptomyces sp. NRRL B-24484

Inhibitors

Inhibitors Comment Organism Structure
additional information not influenced by Triton X-100 and Tween-20 (10% (v/v) Streptomyces sp. NRRL B-24484
SDS the enzyme shows about 45% activity in the presence of 10% (w/v) SDS Streptomyces sp. NRRL B-24484

Organic Solvent Stability

Organic Solvent Comment Organism
Acetone the enzyme retains 100% activity in the presence of 20% (v/v) acetone Streptomyces sp. NRRL B-24484
Ethanol the enzyme retains about 85% activity in the presence of 20% (v/v) ethanol Streptomyces sp. NRRL B-24484
isopropanol the enzyme retains about 65% activity in the presence of 20% (v/v) isopropanol Streptomyces sp. NRRL B-24484
Methanol the enzyme retains about 80% activity in the presence of 20% (v/v) methanol Streptomyces sp. NRRL B-24484

Organism

Organism UniProt Comment Textmining
Streptomyces sp. NRRL B-24484
-
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein
-
Streptomyces sp. NRRL B-24484

Purification (Commentary)

Purification (Comment) Organism
nickel-nitrilotriacetic acid agarose column chromatography and HiTrap Q column chromatography Streptomyces sp. NRRL B-24484

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
galactomannan + H2O
-
Streptomyces sp. NRRL B-24484 ?
-
?
glucomannan + H2O highest activity Streptomyces sp. NRRL B-24484 ?
-
?

Synonyms

Synonyms Comment Organism
GH 134 beta-1,4-mannanase
-
Streptomyces sp. NRRL B-24484
GH134
-
Streptomyces sp. NRRL B-24484

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
40
-
-
Streptomyces sp. NRRL B-24484

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
100
-
the enzyme shows 30% and 60% of the maximal activity after an incubation at 100°C for 120 min and 80°C for 60 min, respectively Streptomyces sp. NRRL B-24484

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
120
-
galactomannan at pH 5.0 and 40°C Streptomyces sp. NRRL B-24484
340
-
glucomannan at pH 5.0 and 40°C Streptomyces sp. NRRL B-24484

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5
-
-
Streptomyces sp. NRRL B-24484

pH Range

pH Minimum pH Maximum Comment Organism
4 6.5 maximally active between pH 4.0 and 6.5 Streptomyces sp. NRRL B-24484

pH Stability

pH Stability pH Stability Maximum Comment Organism
3 10 the enzyme retains more than 80% of the maximum activity after 90 min at 30°C within a pH range of 3.0-10.0 Streptomyces sp. NRRL B-24484